Detection of co-infection of Gardnerella vaginalis and Atopobium vaginae using qualitative PCR: A better predictor of bacterial vaginosis.
Actinobacteria
/ isolation & purification
Adolescent
Adult
Bacteriological Techniques
Coinfection
/ diagnosis
Female
Gardnerella vaginalis
/ isolation & purification
Gram-Positive Bacterial Infections
/ diagnosis
Humans
Polymerase Chain Reaction
/ methods
Sensitivity and Specificity
Vaginosis, Bacterial
/ diagnosis
Young Adult
Atopobium vaginae
Bacterial vaginosis
Gardnerella vaginalis
Qualitative PCR
Journal
Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
17
09
2020
revised:
30
01
2021
accepted:
04
02
2021
pubmed:
15
2
2021
medline:
27
11
2021
entrez:
14
2
2021
Statut:
ppublish
Résumé
The present study aimed to determine the utility of detection of co-infection of Gardnerella vaginalis and Atopobium vaginae using qualitative PCR for diagnosing bacterial vaginosis (BV). Vaginal samples (n = 385) categorized as positive (n = 108) or negative (n = 208) for bacterial vaginosis based on the Nugent scoring system, were analyzed for the presence of G. vaginalis and A. vaginae by conventional PCR. We compared the sensitivity, specificity, positive predictive value, negative predictive value and odds ratio for the detection of each bacterium alone with the combination of the two bacteria for diagnosing BV. The detection of co-infection of the two bacteria demonstrated a sensitivity of 96%, a specificity of 82.9%, a positive predictive value of 68.5%, a negative predictive value of 98.2% with an odds ratio of 116 (CI -32 - 409). In our study, we found a high sensitivity, specificity, negative predictive value and odds ratio for the detection of co-infection of A. vaginae and G. vaginalis for the diagnosis of BV.
Identifiants
pubmed: 33582302
pii: S1075-9964(21)00026-3
doi: 10.1016/j.anaerobe.2021.102343
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102343Informations de copyright
Copyright © 2021. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of competing interest None declared.