Studies to explore the UVA photosensitizing action of 9-phenylacridine in cells by interaction with DNA.
Acridines
/ chemistry
Animals
Antineoplastic Agents
/ chemistry
Apoptosis
/ drug effects
Cattle
Cell Line, Tumor
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
DNA
/ chemistry
Drug Screening Assays, Antitumor
Mice
Osmolar Concentration
Photosensitizing Agents
/ chemistry
Spectrometry, Fluorescence
Thermodynamics
Ultraviolet Rays
9-Phenylacridine
DNA binding parameters
DNA damage
UVA
apoptosis
phototosensitization
Journal
Nucleosides, nucleotides & nucleic acids
ISSN: 1532-2335
Titre abrégé: Nucleosides Nucleotides Nucleic Acids
Pays: United States
ID NLM: 100892832
Informations de publication
Date de publication:
2021
2021
Historique:
pubmed:
16
2
2021
medline:
27
10
2021
entrez:
15
2
2021
Statut:
ppublish
Résumé
Acridine and its derivatives are well known for their DNA binding properties. In this report, we present our findings on evaluating different binding parameters of the interaction of 9-phenylacridine (ACPH) with DNA. Absorption spectroscopic studies including standard and reverse titration, the effects of ionic strength and temperature on titration, and Job plot analysis were done to calculate the binding constant and determine the different thermodynamic parameters and stoichiometry of the binding. Spectrofluorimetry and circular dichroism (CD) spectral titration were also utilized to confirm these findings. The results indicated that ACPH binds to DNA reversibly through non-electrostatic interactions by hydrogen bonding and van der Waals interactions. The binding constant and the number of binding sites were of the order 10
Identifiants
pubmed: 33586599
doi: 10.1080/15257770.2021.1880011
doi:
Substances chimiques
9-phenylacridine
0
Acridines
0
Antineoplastic Agents
0
Photosensitizing Agents
0
DNA
9007-49-2
calf thymus DNA
91080-16-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM