Bioengineering a Light-Responsive Encapsulin Nanoreactor: A Potential Tool for
A549 Cells
Antineoplastic Agents
/ chemistry
Biomedical Engineering
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Drug Screening Assays, Antitumor
Flavoproteins
/ chemistry
Fluorescent Dyes
/ chemistry
Humans
Light
Lung Neoplasms
/ diagnostic imaging
Nanocomposites
/ chemistry
Particle Size
Photochemotherapy
Photosensitizing Agents
/ chemistry
Reactive Oxygen Species
/ analysis
Singlet Oxygen
/ analysis
Spectrometry, Fluorescence
Surface Properties
compartmentalization
encapsulin
nanoreactor
photodynamic therapy
photosensitizer
protein delivery
reactive oxygen species
Journal
ACS applied materials & interfaces
ISSN: 1944-8252
Titre abrégé: ACS Appl Mater Interfaces
Pays: United States
ID NLM: 101504991
Informations de publication
Date de publication:
24 Feb 2021
24 Feb 2021
Historique:
pubmed:
16
2
2021
medline:
6
3
2021
entrez:
15
2
2021
Statut:
ppublish
Résumé
Encapsulins, a prokaryotic class of self-assembling protein nanocompartments, are being re-engineered to serve as "nanoreactors" for the augmentation or creation of key biochemical reactions. However, approaches that allow encapsulin nanoreactors to be functionally activated with spatial and temporal precision are lacking. We report the construction of a light-responsive encapsulin nanoreactor for "on demand" production of reactive oxygen species (ROS). Herein, encapsulins were loaded with the fluorescent flavoprotein mini-singlet oxygen generator (miniSOG), a biological photosensitizer that is activated by blue light to generate ROS, primarily singlet oxygen (
Identifiants
pubmed: 33586952
doi: 10.1021/acsami.0c21141
doi:
Substances chimiques
Antineoplastic Agents
0
Flavoproteins
0
Fluorescent Dyes
0
Photosensitizing Agents
0
Reactive Oxygen Species
0
Singlet Oxygen
17778-80-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM