Electron dose rate and oxygen depletion protect zebrafish embryos from radiation damage.
Electron flash effect
Normal tissue toxicity
Oxygen depletion
Zebrafish embryo
Journal
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology
ISSN: 1879-0887
Titre abrégé: Radiother Oncol
Pays: Ireland
ID NLM: 8407192
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
28
10
2020
revised:
29
01
2021
accepted:
01
02
2021
pubmed:
16
2
2021
medline:
21
5
2021
entrez:
15
2
2021
Statut:
ppublish
Résumé
In consequence of a previous study, where no protecting proton Flash effect was found for zebrafish embryos, potential reasons and requirements for inducing a Flash effect should be investigated with higher pulse dose rate and partial oxygen pressure (pO The experiments were performed at the research electron accelerator ELBE, whose variable pulse structure enables dose delivery as electron Flash and quasi-continuously (reference irradiation). Zebrafish embryos were irradiated with ~26 Gy either continuously at a dose rate of ~6.7 Gy/min (reference) or by 1441 electron pulses within 111 µs at a pulse dose rate of 10 A protective Flash effect was seen for most endpoints ranging from 4 % less reduction in embryo length to about 20-25% less embryos with spinal curvature and pericardial edema, relative to reference irradiation. The reduction of pO The Flash experiment at ELBE showed that the zebrafish embryo model is appropriate for studying the radiobiological response of high dose rate irradiation. The applied high pulse dose rate was confirmed as important beam parameter as well as the pivotal role of pO
Sections du résumé
BACKGROUND AND PURPOSE
In consequence of a previous study, where no protecting proton Flash effect was found for zebrafish embryos, potential reasons and requirements for inducing a Flash effect should be investigated with higher pulse dose rate and partial oxygen pressure (pO
MATERIALS AND METHODS
The experiments were performed at the research electron accelerator ELBE, whose variable pulse structure enables dose delivery as electron Flash and quasi-continuously (reference irradiation). Zebrafish embryos were irradiated with ~26 Gy either continuously at a dose rate of ~6.7 Gy/min (reference) or by 1441 electron pulses within 111 µs at a pulse dose rate of 10
RESULTS
A protective Flash effect was seen for most endpoints ranging from 4 % less reduction in embryo length to about 20-25% less embryos with spinal curvature and pericardial edema, relative to reference irradiation. The reduction of pO
CONCLUSION
The Flash experiment at ELBE showed that the zebrafish embryo model is appropriate for studying the radiobiological response of high dose rate irradiation. The applied high pulse dose rate was confirmed as important beam parameter as well as the pivotal role of pO
Identifiants
pubmed: 33587970
pii: S0167-8140(21)06049-7
doi: 10.1016/j.radonc.2021.02.003
pii:
doi:
Substances chimiques
Protons
0
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7-12Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.