Evaluation of the diagnostic potential of recombinant leptospiral OMP A-like protein (Loa22) and transmembrane (OmpL37) protein in latex agglutination test for serodiagnosis of leptospirosis in animals.
Animals
Antibodies, Bacterial
/ blood
Antibodies, Protozoan
/ blood
Antigens, Bacterial
/ genetics
Bacterial Outer Membrane Proteins
/ immunology
Cattle
Cattle Diseases
/ diagnosis
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
/ methods
Immunoblotting
Latex Fixation Tests
/ methods
Leptospira interrogans
/ immunology
Leptospirosis
/ diagnosis
Recombinant Proteins
/ genetics
Serologic Tests
/ methods
Zoonoses
/ diagnosis
Leptospira
LAT: Animals
Loa22 (LIC10191)
OmpL37 (LIC12263)
leptospirosis
recombinant proteins
serodiagnosis
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
revised:
02
02
2021
received:
05
10
2020
accepted:
11
02
2021
pubmed:
17
2
2021
medline:
28
5
2021
entrez:
16
2
2021
Statut:
ppublish
Résumé
Leptospirosis is a re-emerging zoonotic disease of animals and humans caused by pathogenic Leptospira, which has major public health concerns. The study is aimed to express the recombinant outer membrane protein (OMP) A-like protein (rLoa22) and transmembrane (rOmpL37) protein of Leptospira interrogans serovar Hardjo in the Escherichia coli and their evaluation as a diagnostic antigen in the latex agglutination test (LAT) to detect anti-leptospiral antibodies in the sera of animals. The Loa22 and OmpL37 genes lacking signal peptide coding sequences were individually amplified (522 and 963 bp), by polymerase chain reaction, and directionally cloned into a pETite N-His Kan vector for expression. The expressed purified proteins were characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblot, which confirmed leptospiral specific reactive protein with a molecular weight of ~19 and 36 kDa, respectively. The sensitized latex beads coated with these OM proteins separately were evaluated in LAT using cattle sera of microscopic agglutination test (MAT) confirmed positive (n = 53) and negative (n = 52) cases of leptospirosis. The rLoa22 LAT and rOmpL37 LAT revealed the relative diagnostic sensitivity of 94·34 and 96·23%, diagnostic specificity of 92·31 and 96·15% and accuracy of 93·33 and 96·19%, with the excellent agreement of Cohen's kappa value of 0·87 and 0·92, respectively. After extensive evaluation, this rapid recombinant protein-based field diagnostic test can be applied as a screening test for the detection of anti-leptospiral antibodies in the sera of animals in the field conditions.
Substances chimiques
Antibodies, Bacterial
0
Antibodies, Protozoan
0
Antigens, Bacterial
0
Bacterial Outer Membrane Proteins
0
Recombinant Proteins
0
OMPA outer membrane proteins
149024-69-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
730-740Subventions
Organisme : Indian Council of Medical Research
ID : Leptos/7/2013-ECD-I
Organisme : Indian Council of Medical Research
ID : 80/820/2013-ECD-I
Informations de copyright
© 2021 The Society for Applied Microbiology.
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