In vitro selection of an RNA aptamer yields an interleukin-6/interleukin-6 receptor interaction inhibitor.

Aptamers, Nucleotide / chemistry Base Sequence COVID-19 / complications Cytokine Release Syndrome / drug therapy DNA, Viral / drug effects Humans Interleukin-6 / antagonists & inhibitors Receptors, Interleukin-6 / antagonists & inhibitors SELEX Aptamer Technique COVID-19 Drug Treatment
in vitro selection SELEX aptamer interleukin-6 interleukin-6 receptor

Journal

Bioscience, biotechnology, and biochemistry
ISSN: 1347-6947
Titre abrégé: Biosci Biotechnol Biochem
Pays: England
ID NLM: 9205717

Informations de publication

Date de publication:
24 Apr 2021
Historique:
received: 14 11 2020
accepted: 29 12 2020
pubmed: 17 2 2021
medline: 30 4 2021
entrez: 16 2 2021
Statut: ppublish

Résumé

Interleukin-6 (IL-6) binds to the IL-6 receptor (IL-6R) subunit, related to autoimmune diseases and cytokine storm in COVID-19. In this study, we performed systematic evolution of ligands by exponential enrichment and identified a novel RNA aptamer. This RNA aptamer not only bound to IL-6R with a dissociation constant of 200 n m, but also inhibited the interaction of IL-6R with IL-6.

Identifiants

DOI: 10.1093/bbb/zbaa124 PMID: 33590853 PMC: PMC7799299
pubmed: 33590853
pii: 6058717
doi: 10.1093/bbb/zbaa124
pmc: PMC7799299
doi:

Substances chimiques

Aptamers, Nucleotide 0
DNA, Viral 0
IL6 protein, human 0
IL6R protein, human 0
Interleukin-6 0
Receptors, Interleukin-6 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1170-1174

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.

Auteurs

Takehiro Ando (T)

Department of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.

Mizuki Yamamoto (M)

Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.

Yukio Takamori (Y)

Department of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.

Keita Tsukamoto (K)

Department of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.

Daisuke Fuji (D)

Department of Biotechnology, Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.

Takashi Kawakami (T)

Faculty of Life and Environmental Sciences, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, Kofu, Yamanashi, Japan.
JST, PRESTO, Kawaguchi, Saitama, Japan.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Choc › Syndrome de réponse inflammatoire généralisée › Syndrome de libération de cytokines › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN viral › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Interleukines › Interleukine-6 › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteurs aux cytokines › Récepteurs aux interleukines › Récepteurs à l'interleukine-6 › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Techniques de chimie combinatoire › Technique SELEX › In vitro selection of an RNA aptamer yields an...
questionsmedicales.fr › Thérapeutique › Traitement médicamenteux › Traitements médicamenteux de la COVID-19 › In vitro selection of an RNA aptamer yields an...