Effects of leukoreduction on storage lesions in whole blood and blood components of dogs.
blood
dog
leukoreduction
storage lesions
transfusion
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
revised:
11
01
2021
received:
17
09
2020
accepted:
15
01
2021
pubmed:
17
2
2021
medline:
29
6
2021
entrez:
16
2
2021
Statut:
ppublish
Résumé
Leukoreduction is a routine procedure in human transfusion medicine but is uncommon in veterinary. To evaluate the effect of leukoreduction on the quality of canine whole blood (WB) and blood products during storage. Ten canine blood donors. This is a case series study. An amount of 450 mL of blood was collected from each dog. Five WB and 5 packed red blood cells (pRBC) bags were divided into 2 units each: leukoreduced (LR) and non-leukoreduced (nLR). RBC count, erythrocytes' mean osmotic fragility (MOF), 2,3-diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), percentage of hemolysis, potassium (K), lactate, glucose, and cytokines were measured weekly from day of donation (T0) to day 35 (T35); pH, coagulation times, and clotting factors were evaluated at T0 and T35 from WB and in fresh frozen plasma after 1 year of storage. Leukoreduction showed positive effects on lactate (T35: LR WB 14.42 mmol/L SD 2.71, nLR WB 22.42 mmol/L SD 1.86, LR pRBC 20.88 mmol/L SD 2.65, nLR pRBC 36.81 mmol/L SD 2.34; P < .0001), pH (T35: LR WB 6.88 SD 0.16, nLR WB 6.69 SD 0.20, P = .02; LR pRBC 6.57 SD 0.23, nLR pRBC 6.22 SD 0.11; P < .001), and K (LR pRBC 4.08 mmol/L SD 0.88, nLR pRBC 5.48 mmol/L SD 0.90; P < .001). Increasing values of IL8 were observed in nLR units during storage (T0: 4167 ± 11 888 pg/mL; T35: 6367 ± 11 612 pg/mL). LR blood units are recommended to critically ill dogs with marked inflammatory conditions.
Sections du résumé
BACKGROUND
BACKGROUND
Leukoreduction is a routine procedure in human transfusion medicine but is uncommon in veterinary.
OBJECTIVES
OBJECTIVE
To evaluate the effect of leukoreduction on the quality of canine whole blood (WB) and blood products during storage.
ANIMALS
METHODS
Ten canine blood donors.
METHODS
METHODS
This is a case series study. An amount of 450 mL of blood was collected from each dog. Five WB and 5 packed red blood cells (pRBC) bags were divided into 2 units each: leukoreduced (LR) and non-leukoreduced (nLR). RBC count, erythrocytes' mean osmotic fragility (MOF), 2,3-diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), percentage of hemolysis, potassium (K), lactate, glucose, and cytokines were measured weekly from day of donation (T0) to day 35 (T35); pH, coagulation times, and clotting factors were evaluated at T0 and T35 from WB and in fresh frozen plasma after 1 year of storage.
RESULTS
RESULTS
Leukoreduction showed positive effects on lactate (T35: LR WB 14.42 mmol/L SD 2.71, nLR WB 22.42 mmol/L SD 1.86, LR pRBC 20.88 mmol/L SD 2.65, nLR pRBC 36.81 mmol/L SD 2.34; P < .0001), pH (T35: LR WB 6.88 SD 0.16, nLR WB 6.69 SD 0.20, P = .02; LR pRBC 6.57 SD 0.23, nLR pRBC 6.22 SD 0.11; P < .001), and K (LR pRBC 4.08 mmol/L SD 0.88, nLR pRBC 5.48 mmol/L SD 0.90; P < .001). Increasing values of IL8 were observed in nLR units during storage (T0: 4167 ± 11 888 pg/mL; T35: 6367 ± 11 612 pg/mL).
CONCLUSION AND CLINICAL IMPORTANCE
CONCLUSIONS
LR blood units are recommended to critically ill dogs with marked inflammatory conditions.
Identifiants
pubmed: 33591603
doi: 10.1111/jvim.16039
pmc: PMC7995433
doi:
Substances chimiques
Cytokines
0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
936-945Subventions
Organisme : Italian Ministry of Health
ID : IZS VE 04/15 VE
Informations de copyright
© 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC. on behalf of the American College of Veterinary Internal Medicine.
Références
J Feline Med Surg. 2016 Jun;18(6):462-70
pubmed: 26023192
Am J Vet Res. 2019 Sep;80(9):846-851
pubmed: 31449444
Transfusion. 2014 Aug;54(8):1935-44
pubmed: 24635475
J Vet Emerg Crit Care (San Antonio). 2014 May-Jun;24(3):259-63
pubmed: 24697952
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6858-62
pubmed: 6947260
Vet Anaesth Analg. 2014 May;41(3):233-42
pubmed: 24576208
J Vet Intern Med. 2021 Mar;35(2):936-945
pubmed: 33591603
J Vet Intern Med. 2020 Sep;34(5):1894-1902
pubmed: 32881076
Luminescence. 2003 May-Jun;18(3):173-81
pubmed: 12701093
Vox Sang. 2002 May;82(4):191-7
pubmed: 12047513
Vox Sang. 2014 Jul;107(1):1-9
pubmed: 24446817
Front Vet Sci. 2019 May 17;6:149
pubmed: 31157246
Vet Clin Pathol. 2014 Sep;43(3):362-70
pubmed: 25135622
J Vet Intern Med. 2000 Jul-Aug;14(4):412-7
pubmed: 10935891
Blood Transfus. 2017 Sep;15(5):447-455
pubmed: 28488959
Free Radic Biol Med. 2016 Jul;96:152-65
pubmed: 27094493
Am J Clin Pathol. 2013 Jan;139(1):110-6
pubmed: 23270906
J Vet Intern Med. 2018 May;32(3):1051-1059
pubmed: 29635743
Clin Chem. 2007 Feb;53(2):318-25
pubmed: 17185366
J Vet Sci. 2019 Jan 31;20(1):72-78
pubmed: 30541183
Transfusion. 2009 Dec;49(12):2599-603
pubmed: 20163690
Scand J Trauma Resusc Emerg Med. 2009 Aug 13;17:35
pubmed: 19678943
Anesthesiology. 2011 Sep;115(3):635-49
pubmed: 21792054
J Small Anim Pract. 2017 Dec;58(12):678-684
pubmed: 28741656
Vox Sang. 2000;79(3):151-5
pubmed: 11111233
J Vet Intern Med. 1988 Jul-Sep;2(3):126-32
pubmed: 3225806
Ann Med Health Sci Res. 2015 May-Jun;5(3):185-8
pubmed: 26097760
Am J Vet Res. 2015 Nov;76(11):969-74
pubmed: 26512542
J Vet Intern Med. 1997 Jan-Feb;11(1):5-8
pubmed: 9132483
Cell Biochem Biophys. 2013 Mar;65(2):145-57
pubmed: 22903352
Vox Sang. 2003 Nov;85(4):253-61
pubmed: 14633250
Transfusion. 1995 Mar;35(3):199-203
pubmed: 7878710
Transfus Med Rev. 2020 Oct;34(4):234-241
pubmed: 33051112
J Vet Emerg Crit Care (San Antonio). 2015 Mar-Apr;25(2):187-99
pubmed: 25428860
J Small Anim Pract. 2016 Nov;57(11):637-643
pubmed: 27739591
Hematol Transfus Cell Ther. 2020 Apr - Jun;42(2):134-138
pubmed: 31439519
Transfusion. 2020 May;60(5):1042-1049
pubmed: 32187700
Asian J Transfus Sci. 2007 Jul;1(2):47-51
pubmed: 21938232
Transfus Med Rev. 2002 Jan;16(1):46-60
pubmed: 11788929