Integrative proteomics identifies thousands of distinct, multi-epitope, and high-affinity nanobodies.

Epitopes / genetics Humans Proteomics / methods Single-Domain Antibodies / genetics
antibody immune response antibody technology nanobody structural modeling structural proteomics

Journal

Cell systems
ISSN: 2405-4720
Titre abrégé: Cell Syst
Pays: United States
ID NLM: 101656080

Informations de publication

Date de publication:
17 03 2021
Historique:
received: 01 06 2020
revised: 13 10 2020
accepted: 20 01 2021
pubmed: 17 2 2021
medline: 15 12 2021
entrez: 16 2 2021
Statut: ppublish

Résumé

The antibody immune response is essential for the survival of mammals. However, we still lack a systematic understanding of the antibody repertoire. Here, we developed a proteomic strategy to survey, at an unprecedented scale, the landscape of antigen-engaged, circulating camelid heavy-chain antibodies, whose minimal binding fragments are called V

Identifiants

DOI: 10.1016/j.cels.2021.01.003 PMID: 33592195 PMC: PMC7979497
pubmed: 33592195
pii: S2405-4712(21)00037-5
doi: 10.1016/j.cels.2021.01.003
pmc: PMC7979497
mid: NIHMS1667680
pii:
doi:

Substances chimiques

Epitopes 0
Single-Domain Antibodies 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

220-234.e9

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI163011
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM137905
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

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Auteurs

Yufei Xiang (Y)

Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA.

Zhe Sang (Z)

Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA; University of Pittsburgh, Carnegie Mellon University Program for Computational Biology, Pittsburgh, PA, USA.

Lirane Bitton (L)

School of Computer Science and Engineering, Institute of Life Sciences, The Hebrew University of Jerusalem, Israel.

Jianquan Xu (J)

Departments of Medicine and Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.

Yang Liu (Y)

Departments of Medicine and Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.

Dina Schneidman-Duhovny (D)

School of Computer Science and Engineering, Institute of Life Sciences, The Hebrew University of Jerusalem, Israel. Electronic address: dina.schneidman@mail.huji.ac.il.

Yi Shi (Y)

Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA; University of Pittsburgh, Carnegie Mellon University Program for Computational Biology, Pittsburgh, PA, USA. Electronic address: yi.shi@pitt.edu.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Antigènes › Épitopes › Integrative proteomics identifies thousands of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Integrative proteomics identifies thousands of...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Integrative proteomics identifies thousands of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Fragments d'immunoglobuline › Fragments Fab d'immunoglobuline › Anticorps à domaine unique › Integrative proteomics identifies thousands of...