Genetic regulation and heritability of miRNA and mRNA expression link to phosphorus utilization and gut microbiome.


Journal

Open biology
ISSN: 2046-2441
Titre abrégé: Open Biol
Pays: England
ID NLM: 101580419

Informations de publication

Date de publication:
02 2021
Historique:
entrez: 17 2 2021
pubmed: 18 2 2021
medline: 4 2 2022
Statut: ppublish

Résumé

Improved utilization of phytates and mineral phosphorus (P) in monogastric animals contributes significantly to preserving the finite resource of mineral P and mitigating environmental pollution. In order to identify pathways and to prioritize candidate genes related to P utilization (PU), the genomic heritability of 77 and 80 trait-dependent expressed miRNAs and mRNAs in 482 Japanese quail were estimated and eQTL (expression quantitative trait loci) were detected. In total, 104 miR-eQTL (microRNA expression quantitative traits loci) were associated with SNP markers (false discovery rate less than 10%) including 41 eQTL of eight miRNAs. Similarly, 944 mRNA-eQTL were identified at the 5% False discovery rate threshold, with 573 being cis-eQTL of 36 mRNAs. High heritabilities of miRNA and mRNA expression coincide with highly significant eQTL. Integration of phenotypic data with transcriptome and microbiome data of the same animals revealed genetic regulated mRNA and miRNA transcripts (SMAD3, CAV1, ENNPP6, ATP2B4, miR-148a-3p, miR-146b-5p, miR-16-5p, miR-194, miR-215-5p, miR-199-3p, miR-1388a-3p) and microbes (

Identifiants

pubmed: 33593158
doi: 10.1098/rsob.200182
pmc: PMC8061690
doi:

Substances chimiques

Avian Proteins 0
MicroRNAs 0
RNA, Messenger 0
Phosphorus 27YLU75U4W

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

200182

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Auteurs

Siriluck Ponsuksili (S)

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.

Michael Oster (M)

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.

Henry Reyer (H)

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.

Frieder Hadlich (F)

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.

Nares Trakooljul (N)

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.

Markus Rodehutscord (M)

Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany.

Amélia Camarinha-Silva (A)

Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany.

Jörn Bennewitz (J)

Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany.

Klaus Wimmers (K)

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.
Faculty of Agricultural and Environmental Sciences, University Rostock, 18059 Rostock, Germany.

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