Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples.
Influenza
Rapid detection
TRC method; RT-PCR
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
ISSN: 1435-4373
Titre abrégé: Eur J Clin Microbiol Infect Dis
Pays: Germany
ID NLM: 8804297
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
30
09
2020
accepted:
09
02
2021
pubmed:
18
2
2021
medline:
31
8
2021
entrez:
17
2
2021
Statut:
ppublish
Résumé
We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.
Identifiants
pubmed: 33594599
doi: 10.1007/s10096-021-04193-7
pii: 10.1007/s10096-021-04193-7
pmc: PMC7885976
doi:
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1743-1748Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
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