ppGpp influences protein protection, growth and photosynthesis in Phaeodactylum tricornutum.
Phaeodactylum tricornutum
chloroplast
diatoms
lipid droplets
photosynthesis
ppGpp
proteome
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
25
08
2020
accepted:
08
02
2021
pubmed:
18
2
2021
medline:
15
5
2021
entrez:
17
2
2021
Statut:
ppublish
Résumé
Chloroplasts retain elements of a bacterial stress response pathway that is mediated by the signalling nucleotides guanosine penta- and tetraphosphate ((p)ppGpp). In the model flowering plant Arabidopsis, ppGpp acts as a potent regulator of plastid gene expression and influences photosynthesis, plant growth and development. However, little is known about ppGpp metabolism or its evolution in other photosynthetic eukaryotes. Here, we studied the function of ppGpp in the diatom Phaeodactylum tricornutum using transgenic lines containing an inducible system for ppGpp accumulation. We used these lines to investigate the effects of ppGpp on growth, photosynthesis, lipid metabolism and protein expression. We demonstrate that ppGpp accumulation reduces photosynthetic capacity and promotes a quiescent-like state with reduced proliferation and ageing. Strikingly, using nontargeted proteomics, we discovered that ppGpp accumulation also leads to the coordinated upregulation of a protein protection response in multiple cellular compartments. Our findings highlight the importance of ppGpp as a fundamental regulator of chloroplast function across different domains of life, and lead to new questions about the molecular mechanisms and roles of (p)ppGpp signalling in photosynthetic eukaryotes.
Identifiants
pubmed: 33595847
doi: 10.1111/nph.17286
pmc: PMC8252717
doi:
Substances chimiques
Guanosine Tetraphosphate
33503-72-9
Guanosine Pentaphosphate
38918-96-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1517-1532Informations de copyright
© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.
Références
Curr Biol. 2008 Jun 24;18(12):895-9
pubmed: 18538570
EMBO J. 2004 Nov 10;23(22):4473-83
pubmed: 15496987
Sci Rep. 2019 Nov 27;9(1):17682
pubmed: 31776430
Nature. 2011 May 12;473(7346):203-7
pubmed: 21562560
J Plant Res. 2015 May;128(3):511-8
pubmed: 25752614
Sci Rep. 2016 May 05;6:25494
pubmed: 27147218
Photosynth Res. 2018 Mar;135(1-3):203-211
pubmed: 28039566
FEMS Microbiol Rev. 2010 Sep;34(5):797-827
pubmed: 20636484
Mol Plant Pathol. 2018 Mar;19(3):634-646
pubmed: 28220595
Plant Signal Behav. 2014;9(10):e972874
pubmed: 25482768
Philos Trans R Soc Lond B Biol Sci. 2017 Sep 5;372(1728):
pubmed: 28717027
Philos Trans R Soc Lond B Biol Sci. 2017 Sep 5;372(1728):
pubmed: 28717016
Nat Microbiol. 2018 Nov;3(11):1304-1313
pubmed: 30275513
Plant J. 2012 Jun;70(6):1004-14
pubmed: 22332784
Plant J. 2015 Feb;81(3):519-28
pubmed: 25438865
Plant Physiol. 2015 Jan;167(1):118-36
pubmed: 25489020
FEBS J. 2008 Mar;275(6):1248-59
pubmed: 18266760
Talanta. 2020 Nov 1;219:121261
pubmed: 32887152
Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1767-72
pubmed: 23297242
Biochimie. 2020 Feb;169:12-17
pubmed: 31265860
Can J Microbiol. 1962 Apr;8:229-39
pubmed: 13902807
Plant Cell. 2014 May 30;26(5):2201-2222
pubmed: 24879428
Nat Plants. 2019 Aug;5(8):890-901
pubmed: 31358960
Nat Commun. 2017 Jun 20;8:15885
pubmed: 28631733
Photosynth Res. 2006 Jul;89(1):27-41
pubmed: 16763878
J Cell Sci. 2002 Nov 1;115(Pt 21):4061-9
pubmed: 12356911
Nat Rev Microbiol. 2015 May;13(5):298-309
pubmed: 25853779
J Biol Chem. 1991 Feb 25;266(6):3760-7
pubmed: 1899866
Mar Drugs. 2018 Jan 22;16(1):
pubmed: 29361768
BMC Biotechnol. 2011 Jan 21;11:7
pubmed: 21255402
Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4320-4
pubmed: 15010537
Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4867-76
pubmed: 27486247
Elife. 2017 May 12;6:
pubmed: 28498102
Science. 2000 Oct 13;290(5490):291-6
pubmed: 11030643
Photosynth Res. 2018 Mar;135(1-3):299-308
pubmed: 28536785
PLoS One. 2011;6(8):e23479
pubmed: 21858139
Front Plant Sci. 2020 Jul 16;11:1033
pubmed: 32765548
J Cell Biol. 1985 Mar;100(3):965-73
pubmed: 3972906
Algal Res. 2016 Sep;18:213-224
pubmed: 27812494
Plant J. 2018 Apr;94(2):327-339
pubmed: 29441718
Philos Trans R Soc Lond B Biol Sci. 2017 Sep 5;372(1728):
pubmed: 28717012
J Control Release. 2018 Jul 10;281:70-83
pubmed: 29772290
Trends Biotechnol. 2014 Mar;32(3):117-24
pubmed: 24529448
Philos Trans R Soc Lond B Biol Sci. 2017 Sep 5;372(1728):
pubmed: 28717023
J Basic Microbiol. 1985;25(2):127-40
pubmed: 4009429
Plant Cell. 2016 Mar;28(3):661-79
pubmed: 26908759
Mol Microbiol. 2016 Aug;101(4):531-44
pubmed: 27149325
Biotechnol Biofuels. 2018 Apr 23;11:120
pubmed: 29713379
J Bacteriol. 1995 Aug;177(15):4224-9
pubmed: 7635809
Science. 2019 Aug 2;365(6452):
pubmed: 31371578
Elife. 2019 Oct 15;8:
pubmed: 31612858
Nat Rev Mol Cell Biol. 2013 Oct;14(10):630-42
pubmed: 24026055
Methods Mol Biol. 2007;390:257-67
pubmed: 17951693
Mar Biotechnol (NY). 1999 May;1(3):239-251
pubmed: 10383998
J Exp Bot. 2018 May 19;69(11):2797-2807
pubmed: 29281108
Nature. 2008 Nov 13;456(7219):239-44
pubmed: 18923393
Mol Cell Proteomics. 2014 Sep;13(9):2513-26
pubmed: 24942700
Photosynth Res. 2012 Mar;111(1-2):245-57
pubmed: 21327535
J Plant Res. 2017 Jul;130(4):625-634
pubmed: 28303404
Plant J. 2015 Jun;82(6):1030-1041
pubmed: 25976841
Gene. 2007 Dec 30;406(1-2):23-35
pubmed: 17658702
Mol Microbiol. 2001 Jan;39(1):136-44
pubmed: 11123695
PLoS Genet. 2017 Sep 22;13(9):e1007022
pubmed: 28937985
New Phytol. 2020 Nov;228(3):946-958
pubmed: 32535932
Plant Cell Environ. 2016 Apr;39(4):834-47
pubmed: 26477535
Plant Cell Physiol. 2021 Feb 4;61(12):2077-2086
pubmed: 33089303
J Biol Chem. 2014 May 30;289(22):15631-41
pubmed: 24722991
Nat Plants. 2015 Nov 09;1:15167
pubmed: 27251712
Plant Physiol. 2006 May;141(1):75-84
pubmed: 16531478
Appl Environ Microbiol. 2012 Aug;78(15):5440-3
pubmed: 22610439
J Biol Chem. 2012 Jun 15;287(25):21034-44
pubmed: 22547063
PeerJ. 2016 Aug 25;4:e2344
pubmed: 27635322