Role of reptiles and associated arthropods in the epidemiology of rickettsioses: A one health paradigm.
Adult
Aged
Anaplasma phagocytophilum
Anaplasmataceae
/ genetics
Animals
Arthropods
/ microbiology
Coxiella burnetii
Europe
Female
Humans
Italy
Ixodes
/ microbiology
Male
Middle Aged
One Health
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Reptiles
/ microbiology
Rickettsia
Rickettsia Infections
/ epidemiology
Snakes
Young Adult
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
16
09
2020
accepted:
29
12
2020
entrez:
17
2
2021
pubmed:
18
2
2021
medline:
23
6
2021
Statut:
epublish
Résumé
We assessed the presence of Rickettsia spp., Coxiella burnetii and Anaplasma phagocytophilum in reptiles, their ectoparasites and in questing ticks collected in a nature preserve park in southern Italy, as well as in a peri-urban area in another region. We also investigated the exposure to these pathogens in forestry workers, farmers and livestock breeders living or working in the nature preserve park given the report of anecdotal cases of spotted fever rickettsioses. Rickettsia spp. were molecularly detected in Podarcis muralis and Podarcis siculus lizards (i.e., 3.1%), in Ixodes ricinus (up to 87.5%) and in Neotrombicula autumnalis (up to 8.3%) collected from them as well as in I. ricinus collected from the environment (up to 28.4%). Rickettsia monacensis was the most prevalent species followed by Rickettsia helvetica. An undescribed member of the family Anaplasmataceae was detected in 2.4% and 0.8% of the reptiles and ectoparasites, respectively. Sera from human subjects (n = 50) were serologically screened and antibodies to Rickettsia spp. (n = 4; 8%), C. burnetti (n = 8; 16%) and A. phagocytophilum (n = 11; 22%) were detected. Two ticks collected from two forestry workers were positive for spotted fever group (SFG) rickettsiae. Ixodes ricinus is involved in the transmission of SFG rickettsiae (R. monacensis and R. helvetica) in southern Europe and lizards could play a role in the sylvatic cycle of R. monacensis, as amplifying hosts. Meanwhile, N. autumnalis could be involved in the enzootic cycle of some SFG rickettsiae among these animals. People living or working in the southern Italian nature preserve park investigated are exposed to SFG rickettsiae, C. burnetii and A. phagocytophilum.
Identifiants
pubmed: 33596200
doi: 10.1371/journal.pntd.0009090
pii: PNTD-D-20-01669
pmc: PMC7888606
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0009090Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Ticks Tick Borne Dis. 2016 Jul;7(5):1029-1034
pubmed: 27282078
Clin Microbiol Rev. 2017 Jan;30(1):115-190
pubmed: 27856520
Emerg Infect Dis. 2012 Apr;18(4):702-4
pubmed: 22469314
Microbiol Spectr. 2016 Jun;4(3):
pubmed: 27337487
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Vector Borne Zoonotic Dis. 2015 Jul;15(7):404-11
pubmed: 26134933
Exp Appl Acarol. 2012 Oct;58(2):167-74
pubmed: 22610454
Ticks Tick Borne Dis. 2019 Aug;10(5):987-994
pubmed: 31126748
Microb Ecol. 2011 Feb;61(2):245-53
pubmed: 20711724
Parasit Vectors. 2014 Jul 14;7:328
pubmed: 25023709
Biotechniques. 1993 Sep;15(3):532-4, 536-7
pubmed: 7692896
Mol Biol Evol. 1992 Jul;9(4):678-87
pubmed: 1630306
Exp Appl Acarol. 2006;39(3-4):321-9
pubmed: 16821092
Ann N Y Acad Sci. 2005 Dec;1063:1-12
pubmed: 16481485
G Ital Med Lav Ergon. 2007 Jul-Sep;29(3 Suppl):811-2
pubmed: 18409975
J Bacteriol. 1991 Mar;173(5):1576-89
pubmed: 1671856
PLoS One. 2015 Sep 16;10(9):e0135828
pubmed: 26375033
Exp Appl Acarol. 2012 Jun;57(2):199-208
pubmed: 22402940
Clin Microbiol Rev. 2013 Oct;26(4):657-702
pubmed: 24092850
Emerg Infect Dis. 2007 Sep;13(9):1405-7
pubmed: 18252123
Environ Microbiol. 2016 Mar;18(3):988-96
pubmed: 26627444
Clin Vaccine Immunol. 2007 Nov;14(11):1526-8
pubmed: 17913859
Vector Borne Zoonotic Dis. 2012 Dec;12(12):1019-22
pubmed: 23186170
Int J Syst Evol Microbiol. 2020 Jul;70(7):3956-4042
pubmed: 32603289
Parasit Vectors. 2019 Jan 15;12(1):35
pubmed: 30646928
Vector Borne Zoonotic Dis. 2010 Jun;10(5):459-63
pubmed: 19877815
Exp Appl Acarol. 2011 Nov;55(3):293-300
pubmed: 21728058
Int J Occup Med Environ Health. 2015;28(5):901-7
pubmed: 26224501
Trends Parasitol. 2012 Oct;28(10):437-46
pubmed: 22902521
Environ Microbiol Rep. 2016 Oct;8(5):708-714
pubmed: 27336914
Front Public Health. 2014 Dec 01;2:251
pubmed: 25520947
Ticks Tick Borne Dis. 2015 Sep;6(6):711-4
pubmed: 26141751
Vector Borne Zoonotic Dis. 2012 Apr;12(4):269-74
pubmed: 22217177
Trends Parasitol. 2020 Aug;36(8):677-687
pubmed: 32448703
Clin Microbiol Rev. 1997 Oct;10(4):694-719
pubmed: 9336669
Ticks Tick Borne Dis. 2016 Mar;7(2):378-83
pubmed: 26724898
Parasit Vectors. 2015 Jan 15;8:27
pubmed: 25589174
Am J Trop Med Hyg. 2013 May;88(5):937-9
pubmed: 23509125
Vet Parasitol. 2013 Feb 18;192(1-3):253-8
pubmed: 23092681
PLoS One. 2015 Jul 24;10(7):e0133700
pubmed: 26207382
Exp Appl Acarol. 2011 Jan;53(1):95-102
pubmed: 20711803
Emerg Infect Dis. 2018 Dec;24(12):2348-2351
pubmed: 30457527
Future Microbiol. 2009 Apr;4(3):323-39
pubmed: 19327117
Ann N Y Acad Sci. 2005 Dec;1063:187-96
pubmed: 16481513
Parasit Vectors. 2010 May 14;3:42
pubmed: 20470386
Microb Ecol. 2017 Nov;74(4):961-968
pubmed: 28540487
Vector Borne Zoonotic Dis. 2009 Jun;9(3):331-6
pubmed: 19492950
Clin Microbiol Rev. 1999 Oct;12(4):518-53
pubmed: 10515901
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Parasit Vectors. 2009 Sep 04;2(1):41
pubmed: 19732416
Exp Parasitol. 2005 Mar;109(3):176-80
pubmed: 15713449
Parasit Vectors. 2010 Jan 11;3:2
pubmed: 20145730
Biomedica. 2007 Sep;27(3):364-71
pubmed: 18320102
Ticks Tick Borne Dis. 2015 Apr;6(3):330-3
pubmed: 25769386
Parasit Vectors. 2019 Jun 28;12(1):328
pubmed: 31253201
Exp Appl Acarol. 2004;33(1-2):93-102
pubmed: 15285141
Iran J Arthropod Borne Dis. 2011;5(1):1-6
pubmed: 22808404
Appl Environ Microbiol. 2012 May;78(10):3767-9
pubmed: 22407681
Vet Parasitol. 2015 Feb 28;208(1-2):9-13
pubmed: 25579394
Ticks Tick Borne Dis. 2016 Jul;7(5):1052-1056
pubmed: 27365155
Ann Ist Super Sanita. 2015;51(4):284-90
pubmed: 26783214
J Parasitol. 2009 Oct;95(5):1165-70
pubmed: 19281295
Clin Microbiol Rev. 2005 Oct;18(4):719-56
pubmed: 16223955
Ticks Tick Borne Dis. 2019 Jan;10(1):43-50
pubmed: 30197269
J Clin Microbiol. 2004 Jan;42(1):90-8
pubmed: 14715737
Ann Clin Microbiol Antimicrob. 2005 Oct 03;4:15
pubmed: 16202127
Epidemiol Infect. 2020 Jan 20;148:e9
pubmed: 31957632
Parasit Vectors. 2018 Apr 24;11(1):263
pubmed: 29690900