16S rRNA gene sequencing of rectal swab in patients affected by COVID-19.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
16
09
2020
accepted:
31
01
2021
entrez:
17
2
2021
pubmed:
18
2
2021
medline:
17
3
2021
Statut:
epublish
Résumé
COronaVIrus Disease-2019 (COVID-19) is a pandemic respiratory infection caused by a new betacoronavirus, the Severe Acute Respiratory Syndrome-CoronaVirus-2 (SARS-CoV-2). Few data are reported on the gut microbiota in COVID-19 patients. 16S rRNA gene sequencing was performed to reveal an altered composition of the gut microbiota in patients with COVID-19 pneumonia admitted in intensive care unit (ICU) (i-COVID19), or in infectious disease wards (w-COVID19) as compared to controls (CTRL). i-COVID19 patients showed a decrease of Chao1 index as compared to CTRL and w-COVID19 patients indicating that patients in ICU displayed a lower microbial richness while no change was observed as for Shannon Index. At the phylum level, an increase of Proteobacteria was detected in w-COVID19 patients as compared to CTRL. A decrease of Fusobacteria and Spirochetes has been found, with the latter decreased in i-COVID19 patients as compared to CTRL. Significant changes in gut microbial communities in patients with COVID-19 pneumonia with different disease severity compared to CTRL have been identified. Our preliminary data may provide valuable information and promising biomarkers for the diagnosis of the disease and, when validated in larger cohort, it could facilitate the stratification of patients based on the microbial signature.
Identifiants
pubmed: 33596245
doi: 10.1371/journal.pone.0247041
pii: PONE-D-20-29220
pmc: PMC7888592
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0247041Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Lancet. 2020 Feb 22;395(10224):565-574
pubmed: 32007145
New Microbiol. 2016 Jan;39(1):1-12
pubmed: 26922981
Front Cell Infect Microbiol. 2015 Nov 20;5:84
pubmed: 26636046
mSystems. 2017 Jul 18;2(4):
pubmed: 28761933
Front Microbiol. 2020 Feb 25;11:301
pubmed: 32158441
Nat Rev Microbiol. 2017 Jan;15(1):55-63
pubmed: 27694885
Pediatr Res. 2019 May;85(6):895-903
pubmed: 30758325
Cell Microbiol. 2018 Dec;20(12):e12966
pubmed: 30329198
Dig Dis. 2016;34(3):260-8
pubmed: 27028893
Cell Rep. 2019 Jul 2;28(1):245-256.e4
pubmed: 31269444
Curr Opin Microbiol. 2013 Jun;16(3):255-61
pubmed: 23831042
Front Microbiol. 2015 Oct 07;6:1085
pubmed: 26500629
Nat Commun. 2013;4:2106
pubmed: 23820884
Science. 2011 Oct 14;334(6053):249-52
pubmed: 21998395
Nature. 2003 Nov 27;426(6965):450-4
pubmed: 14647384
Gut. 2020 Jun;69(6):1141-1143
pubmed: 32102928
Annu Rev Virol. 2014;1:55-69
pubmed: 25821837
J Dig Dis. 2020 Mar;21(3):125-126
pubmed: 32096611
BMC Microbiol. 2017 Mar 31;17(1):78
pubmed: 28359329
Immunol Rev. 2017 Sep;279(1):70-89
pubmed: 28856738
Int J Infect Dis. 2020 Jun;95:363-370
pubmed: 32335340
Sci Rep. 2019 Jul 4;9(1):9673
pubmed: 31273307
mBio. 2020 Feb 18;11(1):
pubmed: 32071269
Gastroenterology. 2020 Sep;159(3):944-955.e8
pubmed: 32442562
Int J Environ Res Public Health. 2019 Oct 23;16(21):
pubmed: 31652705
Gut. 2021 Feb;70(2):276-284
pubmed: 32690600
Ann Am Thorac Soc. 2015 Nov;12 Suppl 2:S150-6
pubmed: 26595731
Int J Biol Sci. 2020 Mar 15;16(10):1753-1766
pubmed: 32226295
Lancet. 2020 Feb 15;395(10223):507-513
pubmed: 32007143
Autoimmun Rev. 2020 Jun;19(6):102538
pubmed: 32268212
JAMA. 2020 May 12;323(18):1843-1844
pubmed: 32159775
Gut. 2020 Jun;69(6):1144-1145
pubmed: 32198152
Emerg Microbes Infect. 2020 Dec;9(1):386-389
pubmed: 32065057
Sci Rep. 2019 Nov 5;9(1):16072
pubmed: 31690798
Microb Genom. 2019 Sep;5(9):
pubmed: 31526447