Major role of IgM in the neutralizing activity of convalescent plasma against SARS-CoV-2.


Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
02 03 2021
Historique:
received: 19 10 2020
revised: 05 01 2021
accepted: 04 02 2021
pubmed: 18 2 2021
medline: 16 3 2021
entrez: 17 2 2021
Statut: ppublish

Résumé

Characterization of the humoral response to SARS-CoV-2, the etiological agent of COVID-19, is essential to help control the infection. The neutralization activity of plasma from patients with COVID-19 decreases rapidly during the first weeks after recovery. However, the specific role of each immunoglobulin isotype in the overall neutralizing capacity is still not well understood. In this study, we select plasma from a cohort of convalescent patients with COVID-19 and selectively deplete immunoglobulin A, M, or G before testing the remaining neutralizing capacity of the depleted plasma. We find that depletion of immunoglobulin M is associated with the most substantial loss of virus neutralization, followed by immunoglobulin G. This observation may help design efficient antibody-based COVID-19 therapies and may also explain the increased susceptibility to SARS-CoV-2 of autoimmune patients receiving therapies that impair the production of immunoglobulin M (IgM).

Identifiants

pubmed: 33596407
pii: S2211-1247(21)00104-2
doi: 10.1016/j.celrep.2021.108790
pmc: PMC7874916
pii:
doi:

Substances chimiques

Antibodies, Neutralizing 0
Antibodies, Viral 0
Immunoglobulin A 0
Immunoglobulin G 0
Immunoglobulin M 0
Spike Glycoprotein, Coronavirus 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

108790

Informations de copyright

Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Romain Gasser (R)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada.

Marc Cloutier (M)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Jérémie Prévost (J)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada.

Corby Fink (C)

Biotherapeutics Research Laboratory, Robarts Research Institute, London, ON NGA 5B7, Canada; Department of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5B7, Canada.

Éric Ducas (É)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Shilei Ding (S)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada.

Nathalie Dussault (N)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Patricia Landry (P)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Tony Tremblay (T)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Audrey Laforce-Lavoie (A)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Antoine Lewin (A)

Héma-Québec, Affaires Médicales et Innovation, Montréal, QC H4R 2W7, Canada; Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada.

Guillaume Beaudoin-Bussières (G)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada.

Annemarie Laumaea (A)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada.

Halima Medjahed (H)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada.

Catherine Larochelle (C)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada; Department of Neurosciences, University of Montreal, Montreal, QC H2X 0A9, Canada.

Jonathan Richard (J)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada.

Gregory A Dekaban (GA)

Biotherapeutics Research Laboratory, Robarts Research Institute, London, ON NGA 5B7, Canada; Department of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5B7, Canada.

Jimmy D Dikeakos (JD)

Department of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5B7, Canada.

Renée Bazin (R)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada. Electronic address: renee.bazin@hema-quebec.qc.ca.

Andrés Finzi (A)

Centre de recherche du CHUM, Montréal, QC H2X 0A9, Canada; Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H2X 0A9, Canada; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada. Electronic address: andres.finzi@umontreal.ca.

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Classifications MeSH