Optically transparent vertical silicon nanowire arrays for live-cell imaging.
Cell–material interface
Glass substrate
Live-cell phase-contrast imaging
Nanowires
Silicon
Journal
Journal of nanobiotechnology
ISSN: 1477-3155
Titre abrégé: J Nanobiotechnology
Pays: England
ID NLM: 101152208
Informations de publication
Date de publication:
17 Feb 2021
17 Feb 2021
Historique:
received:
29
12
2020
accepted:
06
02
2021
entrez:
18
2
2021
pubmed:
19
2
2021
medline:
15
9
2021
Statut:
epublish
Résumé
Programmable nano-bio interfaces driven by tuneable vertically configured nanostructures have recently emerged as a powerful tool for cellular manipulations and interrogations. Such interfaces have strong potential for ground-breaking advances, particularly in cellular nanobiotechnology and mechanobiology. However, the opaque nature of many nanostructured surfaces makes non-destructive, live-cell characterization of cellular behavior on vertically aligned nanostructures challenging to observe. Here, a new nanofabrication route is proposed that enables harvesting of vertically aligned silicon (Si) nanowires and their subsequent transfer onto an optically transparent substrate, with high efficiency and without artefacts. We demonstrate the potential of this route for efficient live-cell phase contrast imaging and subsequent characterization of cells growing on vertically aligned Si nanowires. This approach provides the first opportunity to understand dynamic cellular responses to a cell-nanowire interface, and thus has the potential to inform the design of future nanoscale cellular manipulation technologies.
Identifiants
pubmed: 33596905
doi: 10.1186/s12951-021-00795-7
pii: 10.1186/s12951-021-00795-7
pmc: PMC7890818
doi:
Substances chimiques
Silicon
Z4152N8IUI
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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