Complex precursor structures of cytolytic cupiennins identified in spider venom gland transcriptomes.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 02 2021
17 02 2021
Historique:
received:
12
10
2020
accepted:
05
02
2021
entrez:
18
2
2021
pubmed:
19
2
2021
medline:
22
12
2021
Statut:
epublish
Résumé
Analysis of spider venom gland transcriptomes focuses on the identification of possible neurotoxins, proteins and enzymes. Here, the first comprehensive transcriptome analysis of cupiennins, small linear cationic peptides, also known as cytolytic or antimicrobial peptides, is reported from the venom gland transcriptome of Cupiennius salei by 454- and Illumina 3000 sequencing. Four transcript families with complex precursor structures are responsible for the expression of 179 linear peptides. Within the transcript families, after an anionic propeptide, cationic linear peptides are separated by anionic linkers, which are transcript family specific. The C-terminus of the transcript families is characterized by a linear peptide or truncated linkers with unknown function. A new identified posttranslational processing mechanism explains the presence of the two-chain CsTx-16 family in the venom. The high diversity of linear peptides in the venom of a spider and this unique synthesis process is at least genus specific as verified with Cupiennius getazi.
Identifiants
pubmed: 33597701
doi: 10.1038/s41598-021-83624-z
pii: 10.1038/s41598-021-83624-z
pmc: PMC7889660
doi:
Substances chimiques
Antimicrobial Cationic Peptides
0
Neurotoxins
0
Peptides
0
Spider Venoms
0
cupiennin 1D, Cupiennius salei
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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