Targeting the Active Rhizosphere Microbiome of
BrdU
GCEF
active microbiome
biodiversity-ecosystem functioning
rhizosphere
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2021
2021
Historique:
received:
13
11
2020
accepted:
08
01
2021
entrez:
18
2
2021
pubmed:
19
2
2021
medline:
19
2
2021
Statut:
epublish
Résumé
The relationship between biodiversity and ecosystem functioning (BEF) is a central issue in soil and microbial ecology. To date, most belowground BEF studies focus on the diversity of microbes analyzed by barcoding on total DNA, which targets both active and inactive microbes. This approach creates a bias as it mixes the part of the microbiome currently steering processes that provide actual ecosystem functions with the part not directly involved. Using experimental extensive grasslands under current and future climate, we used the bromodeoxyuridine (BrdU) immunocapture technique combined with pair-end Illumina sequencing to characterize both total and active microbiomes (including both bacteria and fungi) in the rhizosphere of
Identifiants
pubmed: 33597941
doi: 10.3389/fmicb.2021.629169
pmc: PMC7882529
doi:
Types de publication
Journal Article
Langues
eng
Pagination
629169Informations de copyright
Copyright © 2021 Wahdan, Heintz-Buschart, Sansupa, Tanunchai, Wu, Schädler, Noll, Purahong and Buscot.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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