In Vivo and Ex Vivo CT Imaging of Zebrafish Gonads.
Anesthesia
In vivo computed tomography
Phase-contrast CT
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
19
2
2021
pubmed:
20
2
2021
medline:
31
3
2021
Statut:
ppublish
Résumé
3D imaging of the gonads in adult zebrafish in vivo is of great interest, as it allows to follow up on their development and/or the egg development in the same individual over time. Optical-based imaging methods can hardly be applied on the adult zebrafish, due to their limited transparency. In this chapter, we will demonstrate the application of micro computer tomography (CT) imaging for in vivo 3D imaging of the gonads in adult zebrafish. We explain how the limited soft-tissue contrast in CT can be overcome and which X-ray dose levels can be expected using this technique. Moreover, we will use high-resolution microCT to perform ex vivo 3D virtual histology of the adult zebrafish, which allows a simple quantitative analysis of the gonad regions, malformation or alterations in the development of the follicles.
Identifiants
pubmed: 33606228
doi: 10.1007/978-1-0716-0970-5_11
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
129-135Références
Omar M, Rebling J, Wicker K et al (2017) Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy. Light Sci Appl 6:e16186–e16186. https://doi.org/10.1038/lsa.2016.186
pubmed: 30167190
pmcid: 6061890
Holdsworth DW, Thornton MM (2002) Micro-CT in small animal and specimen imaging. Trends Biotechnol 20:S34–S39. https://doi.org/10.1016/S0167-7799(02)02004-8
Brenner DJ, Hall EJ (2007) Computed tomography – an increasing source of radiation exposure. N Engl J Med 357:2277–2284. https://doi.org/10.1056/NEJMra072149
pubmed: 18046031
Carlson SK, Classic KL, Bender CE, Russell SJ (2007) Small animal absorbed radiation dose from serial micro-computed tomography imaging. Mol Imaging Biol 9:78–82
pubmed: 17285239
Vasquez SX, Shah N, Hoberman AM (2013) Small animal imaging and examination by micro-CT. In: Barrow PC (ed) Teratogenicity testing: methods and protocols. Humana, Totowa, NJ, pp 223–231
Valentim AM, Eeden FJ, Strähle U, Olsson IAS (2016) Euthanizing zebrafish legally in Europe: are the approved methods of euthanizing zebrafish appropriate to research reality and animal welfare? EMBO Rep 17:1688–1689. https://doi.org/10.15252/embr.201643153
pubmed: 27797854
pmcid: 5283582