Detection of the Polar Body After Fertilization.

Actin Cytoskeleton / metabolism Animals Cell Nucleus / metabolism Female Fertilization / physiology Fertilization in Vitro / methods Male Meiosis / physiology Oocytes / metabolism Polar Bodies / metabolism Zebrafish / metabolism
DAPI Fertilization Meiosis Phalloidin Polar body Zebrafish

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2021
Historique:
entrez: 19 2 2021
pubmed: 20 2 2021
medline: 31 3 2021
Statut: ppublish

Résumé

The polar body, with haploid DNA, is a small cell produced during the meiosis of an oocyte. Here, we describe the detailed procedures for the detection of the second polar body in zebrafish (Danio rerio) embryos after 10 min post fertilization. A polar body can be easily distinguished as a small dot with a DAPI-stained nucleus surrounded by Phalloidin-labeled F-actin in each fertilized zebrafish embryo.

Identifiants

DOI: 10.1007/978-1-0716-0970-5_13 PMID: 33606230
pubmed: 33606230
doi: 10.1007/978-1-0716-0970-5_13
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

157-167

Références

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Auteurs

Hua Ruan (H)

Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.

Xiaogui Yi (X)

Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China.

Honghui Huang (H)

Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, China. honghuih@126.com.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Cytosquelette d'actine › Detection of the Polar Body After Fertilization.
questionsmedicales.fr › Eucaryotes › Animaux › Detection of the Polar Body After Fertilization.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Noyau de la cellule › Detection of the Polar Body After Fertilization.
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Fécondation › Detection of the Polar Body After Fertilization.
questionsmedicales.fr › Techniques d'investigation › Techniques de reproduction › Techniques de reproduction assistée › Fécondation in vitro › Detection of the Polar Body After Fertilization.
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Méiose › Detection of the Polar Body After Fertilization.
questionsmedicales.fr › Cellules › Cellules germinales › Ovocytes › Detection of the Polar Body After Fertilization.
questionsmedicales.fr › Cellules › Cellules germinales › Ovocytes › Globules polaires › Detection of the Polar Body After Fertilization.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › Detection of the Polar Body After Fertilization.