MC3T3 E1 cell response to mineralized nanofiber shish kebab structures.
3T3 Cells
Acrylic Resins
/ chemistry
Animals
Biocompatible Materials
/ chemistry
Bone and Bones
Calcium Phosphates
/ chemistry
Cell Adhesion
/ drug effects
Cell Proliferation
/ drug effects
Collagen
/ chemistry
Extracellular Matrix
/ chemistry
Humans
Mice
Nanofibers
/ chemistry
Osteoblasts
Osteogenesis
/ drug effects
Polyesters
/ chemistry
Tissue Engineering
Tissue Scaffolds
/ chemistry
bioactivity
biomaterials
block copolymer
mineralization
nanofiber shish kebab
Journal
Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
Titre abrégé: J Biomed Mater Res B Appl Biomater
Pays: United States
ID NLM: 101234238
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
revised:
14
01
2021
received:
23
03
2020
accepted:
01
02
2021
pubmed:
21
2
2021
medline:
11
3
2022
entrez:
20
2
2021
Statut:
ppublish
Résumé
Block copolymers (BCPs) are of growing interest because of their extensive utility in tissue engineering, particularly in biomimetic approaches where multifunctionality is critical. We synthesized polycaprolactone-polyacrylic acid (PCL-b-PAA) BCP and crystallized it onto PCL nanofibers, making BCP nanofiber shish kebab (BCP NFSK) structures. When mineralized in 2× simulated body fluid, BCP NFSK mimic the structure of mineralized collagen fibrils. We hypothesized that the addition of a calcium phosphate layer of graded roughness on the nano-structure of the nanofiber shish kebabs would enhance preosteoblast alkaline phosphatase (ALP) activity, which has been shown to be a critical component in bone matrix formation. The objectives in the study were to investigate the effect of mineralization on cell proliferation and ALP activity, and to also investigate the effect of BCP NFSK periodicity, a structural feature describing the distance between PCL-b-PAA crystals on the nanofiber core, on cell proliferation, and ALP activity. ALP activity of cells cultured on the mineralized BCP NFSK template was significantly higher than the nonmineralized BCP NFSK templates. Interestingly, no statistical difference was observed in ALP activity when the periodic varied, indicating that surface chemistry seemed to play a larger role than the surface roughness.
Substances chimiques
Acrylic Resins
0
Biocompatible Materials
0
Calcium Phosphates
0
Polyesters
0
polycaprolactone
24980-41-4
carbopol 940
4Q93RCW27E
Collagen
9007-34-5
calcium phosphate
97Z1WI3NDX
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1601-1610Informations de copyright
© 2021 Wiley Periodicals LLC.
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