The expression pattern of N-acetyltransferase 1 in healthy human skin.
N-acetyltransferase 1
P-phenylenediamine
contact allergy
human skin
immunofluorescence
Journal
Contact dermatitis
ISSN: 1600-0536
Titre abrégé: Contact Dermatitis
Pays: England
ID NLM: 7604950
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
revised:
05
02
2021
received:
19
07
2019
accepted:
07
02
2021
pubmed:
21
2
2021
medline:
26
11
2021
entrez:
20
2
2021
Statut:
ppublish
Résumé
N-acetyltransferase 1 (NAT1) is an enzyme expressed among others in keratinocytes in human skin. NAT1 is important in the biotransformation of aromatic amines, an important example being p-phenylenediamine (PPD), a hair dye molecule. Unoxidized PPD penetrates the skin and is N-acetylated by NAT1. To investigate in detail the expression pattern of NAT1 in human skin. Cryosections obtained from healthy human skin were stained for NAT1 and expression patterns were observed. NAT1 double stainings were performed with antibodies against different cellular organelles to determine expression patterns. A speckled, granular expression of NAT1 was seen predominantly in the stratum basale. NAT1 was expressed in a cytoplasmic pattern, perinuclear, and in the nucleus. No co-localisation was seen with the selected cellular organelles. Local differences in NAT1 expression patterns were observed between donors and between different biopsies obtained from the same donor. NAT1 is expressed predominantly in the stratum basale and can be found in the cytoplasm, nucleus, and perinuclear in human skin. Further studies should be performed to investigate expression of NAT1 in a larger sample size.
Sections du résumé
BACKGROUND
BACKGROUND
N-acetyltransferase 1 (NAT1) is an enzyme expressed among others in keratinocytes in human skin. NAT1 is important in the biotransformation of aromatic amines, an important example being p-phenylenediamine (PPD), a hair dye molecule. Unoxidized PPD penetrates the skin and is N-acetylated by NAT1.
OBJECTIVES
OBJECTIVE
To investigate in detail the expression pattern of NAT1 in human skin.
MATERIALS AND METHODS
METHODS
Cryosections obtained from healthy human skin were stained for NAT1 and expression patterns were observed. NAT1 double stainings were performed with antibodies against different cellular organelles to determine expression patterns.
RESULT
RESULTS
A speckled, granular expression of NAT1 was seen predominantly in the stratum basale. NAT1 was expressed in a cytoplasmic pattern, perinuclear, and in the nucleus. No co-localisation was seen with the selected cellular organelles. Local differences in NAT1 expression patterns were observed between donors and between different biopsies obtained from the same donor.
CONCLUSIONS
CONCLUSIONS
NAT1 is expressed predominantly in the stratum basale and can be found in the cytoplasm, nucleus, and perinuclear in human skin. Further studies should be performed to investigate expression of NAT1 in a larger sample size.
Identifiants
pubmed: 33609293
doi: 10.1111/cod.13821
pmc: PMC8252542
doi:
Substances chimiques
Isoenzymes
0
Arylamine N-Acetyltransferase
EC 2.3.1.5
N-acetyltransferase 1
EC 2.3.1.5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-6Informations de copyright
© 2021 The Authors. Contact Dermatitis published by John Wiley & Sons Ltd.
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