Ct value-based real time PCR serotyping of Glaesserella parasuis.

Animals Haemophilus Infections / veterinary Haemophilus parasuis / classification Molecular Typing / methods Real-Time Polymerase Chain Reaction / methods Sensitivity and Specificity Serogroup Serotyping / methods Swine Swine Diseases / microbiology
Cycle threshold Glaesserella parasuis Molecular serotyping Real time PCR

Journal

Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469

Informations de publication

Date de publication:
Mar 2021
Historique:
received: 21 10 2020
accepted: 03 02 2021
pubmed: 21 2 2021
medline: 3 9 2021
entrez: 20 2 2021
Statut: ppublish

Résumé

Glaesserella parasuis is the causative agent of Glässer's disease in swine. Serotyping plays an essential role in prevalence investigations and in the development of vaccination strategies for the prevention of this disease. Molecular serotyping based on variation within the capsule loci of the 15 serovars is more accurate and efficient than traditional serological serotyping. To reduce the running time and facilitate ease of data interpretation, we developed a simple and rapid cycle threshold (Ct) value-based real time PCR (qPCR) method for the identification and serotyping of G. parasuis. The qPCR method distinguished between all 15 serovar reference strains of G. parasuis with efficiency values ranging between 85.5 % and 110.4 % and, R

Identifiants

DOI: 10.1016/j.vetmic.2021.109011 PMID: 33610013
pubmed: 33610013
pii: S0378-1135(21)00034-1
doi: 10.1016/j.vetmic.2021.109011
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109011

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Yifang Cui (Y)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Fangfang Guo (F)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Xuwang Cai (X)

State Key Laboratory of Agricultural Microbiology, Division of Animal Infectious Disease, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Xiaoya Cao (X)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Jie Guo (J)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Hongjun Wang (H)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Bing Yang (B)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Hongzhuan Zhou (H)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Xia Su (X)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Patrick J Blackall (PJ)

Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland, 4072, Australia.

Fuzhou Xu (F)

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. Electronic address: fuzhouxu@163.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Pasteurellaceae › Infections à Haemophilus › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pasteurellaceae › Haemophilus › Haemophilus parasuis › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Typage moléculaire › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Sérogroupe › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Sérotypie › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Ct value-based real time PCR serotyping of...
questionsmedicales.fr › Maladies de l'animal › Maladies des porcs › Ct value-based real time PCR serotyping of...