Synthetic biological circuit tested in spaceflight.

Arabidopsis / genetics Arabidopsis Proteins / genetics Estradiol Integrases Plant Leaves / genetics Plants, Genetically Modified RNA Interference RNA, Plant Receptors, Estrogen / genetics Space Flight Synthetic Biology / methods Transcription Factors

Journal

Life sciences in space research
ISSN: 2214-5532
Titre abrégé: Life Sci Space Res (Amst)
Pays: Netherlands
ID NLM: 101632373

Informations de publication

Date de publication:
Feb 2021
Historique:
received: 03 02 2020
revised: 20 08 2020
accepted: 04 09 2020
entrez: 22 2 2021
pubmed: 23 2 2021
medline: 6 8 2021
Statut: ppublish

Résumé

Synthetic biology has potential spaceflight applications yet few if any studies have attempted to translate Earth-based synthetic biology tools into spaceflight. An exogenously inducible biological circuit for protein production in Arabidopsis thaliana, pX7-AtPDSi (Guo et al. 2003), was flown to ISS and functionally investigated. Seedlings were grown in a custom built 1.25 U plant greenhouse. Images recorded during the experiment show that leaves of pX7-AtPDSi seedlings photobleached as designed while wild type Col-0 leaves did not, which reveals that the synthetic circuit led to protein production during spaceflight. Polymerase chain reaction analysis post-flight also confirms that the Cre/LoxP (recombination system) portions of the circuit were functional in spaceflight. The subcomponents of the biological circuit, estrogen-responsive transcription factor XVE, Cre/LoxP DNA recombination system, and RNAi post-transcriptional gene silencing system now have flight heritage and can be incorporated in future designs for space applications. To facilitate future plant studies in space, the full payload design and manufacturing files are made available.

Identifiants

DOI: 10.1016/j.lssr.2020.09.002 PMID: 33612180
pubmed: 33612180
pii: S2214-5524(20)30071-7
doi: 10.1016/j.lssr.2020.09.002
pii:
doi:

Substances chimiques

Arabidopsis Proteins 0
RNA, Plant 0
Receptors, Estrogen 0
Transcription Factors 0
Estradiol 4TI98Z838E
Cre recombinase EC 2.7.7.-
Integrases EC 2.7.7.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

57-65

Informations de copyright

Copyright © 2020. Published by Elsevier B.V.

Auteurs

Rebekah Z Kitto (RZ)

Department of Chemistry, and Henry Eyring Center for Cell and Genome Sciences, University of Utah, Salt Lake City, UT, USA; Department of Chemistry, University of California Berkeley, Berkeley, CA, USA.

Yadvender Dhillon (Y)

International Space University, Strasbourg, France.

James Bevington (J)

International Space University, Strasbourg, France; School of Chemical Engineering, University of New South Wales, Sydney, NSW, Australia.

Mera Horne (M)

Space Science Division, NASA Ames Research Center, Moffett Field, CA, USA.

Philippe Giegé (P)

Institut de Biologie Moléculaire des Plantes-CNRS, Université de Strasbourg, Strasbourg, France.

Laurence Drouard (L)

Institut de Biologie Moléculaire des Plantes-CNRS, Université de Strasbourg, Strasbourg, France.

Dimitri Heintz (D)

Institut de Biologie Moléculaire des Plantes-CNRS, Université de Strasbourg, Strasbourg, France.

Claire Villette (C)

Institut de Biologie Moléculaire des Plantes-CNRS, Université de Strasbourg, Strasbourg, France.

Nicolas Corre (N)

Institut de Biologie Moléculaire des Plantes-CNRS, Université de Strasbourg, Strasbourg, France.

Mathilde Arrivé (M)

Institut de Biologie Moléculaire des Plantes-CNRS, Université de Strasbourg, Strasbourg, France.

Michael J Manefield (MJ)

School of Chemical Engineering, University of New South Wales, Sydney, NSW, Australia; School of Civil and Environmental Engineering, University of New South Wales, Sydney, NSW, Australia.

Robert Bowman (R)

Space Biosciences Division, NASA Ames Research Center, Moffett Field, CA, USA.

Jean-Jacques Favier (JJ)

International Space University, Strasbourg, France.

Barnaby Osborne (B)

International Space University, Strasbourg, France.

Chris Welch (C)

International Space University, Strasbourg, France. Electronic address: chris.welch@isunet.edu.

Christopher P McKay (CP)

Space Science Division, NASA Ames Research Center, Moffett Field, CA, USA. Electronic address: chris.mckay@nasa.gov.

Ming C Hammond (MC)

Department of Chemistry, and Henry Eyring Center for Cell and Genome Sciences, University of Utah, Salt Lake City, UT, USA; Department of Chemistry, University of California Berkeley, Berkeley, CA, USA. Electronic address: ming.hammond@utah.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Hormones gonadiques › Hormones sexuelles stéroïdiennes › Congénères de l'oestradiol › Oestradiol › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Recombinases › Integrases › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Structures de plante › Parties aériennes de plante › Feuilles de plante › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Stades d'organismes › Organismes génétiquement modifiés › Végétaux génétiquement modifiés › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN des plantes › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Récepteurs aux stéroïdes › Récepteurs des oestrogènes › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Technologie, industrie et agriculture › Transports › Aviation › Vol spatial › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Biologie synthétique › Synthetic biological circuit tested in spaceflight.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Synthetic biological circuit tested in spaceflight.