Association between microRNA-527 and glypican-3 in hepatocellular carcinoma.
glypican 3
hepatocellular carcinoma
microRNA
microRNA-527
target gene
Journal
Oncology letters
ISSN: 1792-1074
Titre abrégé: Oncol Lett
Pays: Greece
ID NLM: 101531236
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
27
09
2019
accepted:
19
09
2020
entrez:
22
2
2021
pubmed:
23
2
2021
medline:
23
2
2021
Statut:
ppublish
Résumé
The present study aimed to identify the specific microRNAs (miRNAs/miRs) and their corresponding target genes involved in hepatocellular carcinomas (HCCs). Microarray analysis was performed to examine the miRNA expression profiles of four paired HCC and corresponding non-cancerous (N) liver tissues using 985 miRNA probes. The Human miRNA Target database was used to identify the target genes of differentially expressed miRNAs between the HCC and N tissues. The protein expression levels of target genes in the HCC tissues and cell lines were evaluated using western blotting. miRNA-mediated suppression of target gene expression was evaluated by transiently transfecting the miRNA into the HCC cell lines. Of the 985 miRNAs evaluated, four miRNAs were differentially expressed (three upregulated and one downregulated miRNAs). Of these four miRNAs, miRNA-527 was highly downregulated in the HCC tissues. Glypican-3 (GPC-3) was predicted as a target gene of miRNA-527. Western blotting revealed that GPC-3 protein is highly expressed in the HCC tissues and HCC cell lines compared with N and normal cell lines. Transfection with miR-527 resulted in suppression of GPC-3 protein expression in the Cos7 cells. Furthermore, transfection with miR-527 also inhibited the intrinsic expression of GPC-3 in the Huh-7 cell line. This indicated that miR-527 in the HCC tissues may be an important novel miRNA that targets the GPC-3 gene expression. GPC-3, whose expression is regulated by miR-527, may be involved in the development and progression of HCC.
Identifiants
pubmed: 33613718
doi: 10.3892/ol.2021.12490
pii: OL-0-0-12490
pmc: PMC7856685
doi:
Types de publication
Journal Article
Langues
eng
Pagination
229Informations de copyright
Copyright: © Nomura et al.
Références
Hepatol Res. 2015 Jan;45(2):128-41
pubmed: 25040738
Am J Pathol. 2009 Apr;174(4):1131-8
pubmed: 19264914
Methods Mol Biol. 2019;1970:331-339
pubmed: 30963501
Mol Cancer Res. 2003 Oct;1(12):882-91
pubmed: 14573789
Biochem J. 2008 Mar 15;410(3):503-11
pubmed: 17967162
Cancer Res. 2004 Jun 1;64(11):3753-6
pubmed: 15172979
Med Res Rev. 2018 Mar;38(2):741-767
pubmed: 28621802
Hepatology. 2008 Apr;47(4):1223-32
pubmed: 18307259
PLoS One. 2015 Sep 22;10(9):e0137845
pubmed: 26394044
Hepatobiliary Pancreat Dis Int. 2015 Aug;14(4):361-6
pubmed: 26256079
Exp Cell Res. 2012 Sep 10;318(15):1832-43
pubmed: 22683924
Int J Oncol. 2015 May;46(5):2172-80
pubmed: 25709052
Nat Genet. 2005 May;37(5):495-500
pubmed: 15806104
Pharmacogenet Genomics. 2014 Jun;24(6):283-91
pubmed: 24743544
Int J Oncol. 2015;46(6):2419-30
pubmed: 25823465
Medicine (Baltimore). 2018 Jun;97(24):e11130
pubmed: 29901640
Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11755-60
pubmed: 15284443
J Hepatol. 2010 Jul;53(1):57-66
pubmed: 20447714
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
Acta Biochim Pol. 2012;59(4):467-74
pubmed: 23173124
Dev Cell. 2008 May;14(5):700-11
pubmed: 18477453
Trends Cancer. 2018 Nov;4(11):741-754
pubmed: 30352677
Int J Cancer. 2011 Jan 15;128(2):319-31
pubmed: 20309945
Curr Chem Genom Transl Med. 2018 Jun 29;12:9-26
pubmed: 30069430
Asian Pac J Cancer Prev. 2015;16(9):3595-604
pubmed: 25987009
J Biol Chem. 2011 Nov 18;286(46):39703-14
pubmed: 21903590
Cancer Sci. 2009 Aug;100(8):1403-7
pubmed: 19496787
Cell. 2018 Mar 22;173(1):20-51
pubmed: 29570994
Nat Rev Gastroenterol Hepatol. 2019 Oct;16(10):589-604
pubmed: 31439937
Nucleic Acids Res. 2012 May;40(10):4615-25
pubmed: 22210897
Gastroenterology. 2007 Aug;133(2):647-58
pubmed: 17681183
Hepatology. 2013 Jul;58(1):158-70
pubmed: 23389848
Adv Exp Med Biol. 2015;889:153-77
pubmed: 26659001
FEBS Lett. 2007 May 1;581(9):1805-10
pubmed: 17428475
J Int Med Res. 2011;39(6):2323-9
pubmed: 22289550
Genes Chromosomes Cancer. 2004 Feb;39(2):167-9
pubmed: 14695998
Math Biosci Eng. 2019 May 23;16(5):4607-4621
pubmed: 31499680
Hepatology. 2008 Apr;47(4):1211-22
pubmed: 18318435
World J Hepatol. 2017 Mar 8;9(7):368-384
pubmed: 28321273
Apoptosis. 2008 Oct;13(10):1215-22
pubmed: 18758960
Semin Liver Dis. 2010 Feb;30(1):3-16
pubmed: 20175029
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4
pubmed: 388439
Cancers (Basel). 2019 Sep 10;11(9):
pubmed: 31510063
Bioinformatics. 2003 Jan 22;19(2):185-93
pubmed: 12538238
Cell. 2004 Jan 23;116(2):281-97
pubmed: 14744438
Mamm Genome. 2006 Mar;17(3):189-202
pubmed: 16518686
Oncol Lett. 2019 Jun;17(6):5441-5446
pubmed: 31186763