Noninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgaris.
Journal
International journal of dermatology
ISSN: 1365-4632
Titre abrégé: Int J Dermatol
Pays: England
ID NLM: 0243704
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
revised:
09
09
2020
received:
02
09
2020
accepted:
17
10
2020
pubmed:
23
2
2021
medline:
22
6
2021
entrez:
22
2
2021
Statut:
ppublish
Résumé
The ability of Cutibacterium acnes strains to form biofilms has been correlated with their virulence. This study examined biofilm and skin microbiota in acne patients in order to understand their role in the development of acne lesions. Thin sections of punch biopsy specimens of (i) uninflamed comedones, (ii) inflammatory lesions, and (iii) uninvolved adjacent skin of acne patients were examined. Epiflourescence and confocal laser scanning microscopy were used for biofilm detection, and pyrosequencing with taxonomic classification of 16s rRNA gene amplicons was used for microbiota analysis. Of the 39 skin specimens from patients with mild-moderate acne (n = 13) that were studied, nine (23%) contained biofilm. Among these specimens, biofilm was most frequently detected in comedones (55.6%) and less frequently in inflammatory papules (22.2%) and uninvolved skin (22.2%). Comedones demonstrated the highest mean alpha diversity of all the lesion subtypes. The relative abundance of Staphylococcus was significantly higher in comedones (11.400% ± 12.242%) compared to uninvolved skin (0.073% ± 0.185%, P = 0.024). The microenvironment of the comedone differs from that of inflammatory lesions and unaffected skin. The increased frequency of biofilm in comedones may account for the lack of host inflammatory response to these lesions.
Sections du résumé
BACKGROUND
BACKGROUND
The ability of Cutibacterium acnes strains to form biofilms has been correlated with their virulence.
OBJECTIVE
OBJECTIVE
This study examined biofilm and skin microbiota in acne patients in order to understand their role in the development of acne lesions.
METHODS
METHODS
Thin sections of punch biopsy specimens of (i) uninflamed comedones, (ii) inflammatory lesions, and (iii) uninvolved adjacent skin of acne patients were examined. Epiflourescence and confocal laser scanning microscopy were used for biofilm detection, and pyrosequencing with taxonomic classification of 16s rRNA gene amplicons was used for microbiota analysis.
RESULTS
RESULTS
Of the 39 skin specimens from patients with mild-moderate acne (n = 13) that were studied, nine (23%) contained biofilm. Among these specimens, biofilm was most frequently detected in comedones (55.6%) and less frequently in inflammatory papules (22.2%) and uninvolved skin (22.2%). Comedones demonstrated the highest mean alpha diversity of all the lesion subtypes. The relative abundance of Staphylococcus was significantly higher in comedones (11.400% ± 12.242%) compared to uninvolved skin (0.073% ± 0.185%, P = 0.024).
CONCLUSIONS
CONCLUSIONS
The microenvironment of the comedone differs from that of inflammatory lesions and unaffected skin. The increased frequency of biofilm in comedones may account for the lack of host inflammatory response to these lesions.
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
589-596Subventions
Organisme : Provost Young Investigator Fund of the Johns Hopkins Department of Dermatology
Organisme : American Acne and Rosacea Society
Organisme : University of Maryland School of Medicine
Informations de copyright
© 2020 the International Society of Dermatology.
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