Human Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus.
Animals
COVID-19
/ virology
Cell Line
Chlorocebus aethiops
Enterovirus A, Human
/ pathogenicity
Enterovirus Infections
/ virology
Host Microbial Interactions
/ physiology
Humans
Intestinal Mucosa
/ virology
Intestines
/ virology
Organoids
/ virology
SARS-CoV-2
/ pathogenicity
Vero Cells
Virus Replication
/ physiology
CVA16
EV-A71
SARS-CoV
SARS-CoV-2
intestinal organoids
pathogenicity
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
09 03 2021
09 03 2021
Historique:
received:
18
12
2020
revised:
09
02
2021
accepted:
09
02
2021
pubmed:
25
2
2021
medline:
25
3
2021
entrez:
24
2
2021
Statut:
ppublish
Résumé
Enteroviruses, such as EV-A71 and CVA16, mainly infect the human gastrointestinal tract. Human coronaviruses, including SARS-CoV and SARS-CoV-2, have been variably associated with gastrointestinal symptoms. We aimed to optimize the human intestinal organoids and hypothesize that these optimized intestinal organoids can recapitulate enteric infections of enterovirus and coronavirus. We demonstrate that the optimized human intestinal organoids enable better simulation of the native human intestinal epithelium, and that they are significantly more susceptible to EV-A71 than CVA16. Higher replication of EV-A71 than CVA16 in the intestinal organoids triggers a more vigorous cellular response. However, SARS-CoV and SARS-CoV-2 exhibit distinct dynamics of virus-host interaction; more robust propagation of SARS-CoV triggers minimal cellular response, whereas, SARS-CoV-2 exhibits lower replication capacity but elicits a moderate cellular response. Taken together, the disparate profile of the virus-host interaction of enteroviruses and coronaviruses in human intestinal organoids may unravel the cellular basis of the distinct pathogenicity of these viral pathogens.
Identifiants
pubmed: 33626333
pii: S2213-6711(21)00089-8
doi: 10.1016/j.stemcr.2021.02.009
pmc: PMC7940440
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
493-504Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
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