Novel Technique of Vaginoplasty Developing Normal Vagina, Role of Stemness Markers and Translational Genes.
Absent vagina
Mayer–Rokitansky–Kustner–Hauser
genetics
progenitor cell
vaginoplasty
Journal
Journal of human reproductive sciences
ISSN: 0974-1208
Titre abrégé: J Hum Reprod Sci
Pays: India
ID NLM: 101473512
Informations de publication
Date de publication:
Historique:
received:
18
04
2020
revised:
10
08
2020
accepted:
17
09
2020
entrez:
25
2
2021
pubmed:
26
2
2021
medline:
26
2
2021
Statut:
ppublish
Résumé
To study development of neo-vagina by metaplastic conversion of peritoneum, To identify translational Stemness markers using NANOG/OCT4/SOX2 from serial neo-vaginal mRNA, cDNA and to study role of WNT and HOXA genes in patients undergoing vaginoplasty. 75 MRKH Syndrome women underwent laparoscopic peritoneal vaginoplasty (LPV). Two patients underwent serial neo-vaginal biopsies on day 0, 7-9, 12-14, 21 and 33. Fifteen MRKHS and twelve controls were subjected for neo-vaginal biopsy to detect genes upregulation. Remaining patients were evaluated anatomically and functionally. The translational stemness markers NANOG, OCT4 and SOX2 responsible for neo-vaginal formation were identified. Their appearance, concentration at different stages of conversion were demonstrated. The neo-vagina has shown up-regulation of these translational stemness markers. The study demonstrates expression of the specific genes (WNT4, WNT5A and WNT7A) and their role in formation of the neo-vagina. In the subjects stemness markers (NANOG, OCT4 and SOX2) appeared from day 9 to 14 of the neo-vaginal biopsies and after achieving the peak declined later. Genetic analysis showed low values in HOXA 9,10,11,13 and up-regulation of WNT 4A,5A,7 genes in neo-vagina. Study shows peritoneal metaplastic conversion to normal vagina, identified the translational stemness markers and genes responsible. The neo-vagina has shown up-regulation of these genes. The study demonstrates expression of the specific genes (WNT4, WNT5A and WNT7A) and their role in formation of the neo-vagina. Furthering this research, activating these genes may lead to treatment of developmental defects of Mullerian duct, obviating the need of transplant.
Identifiants
pubmed: 33627980
doi: 10.4103/jhrs.JHRS_68_20
pii: JHRS-13-303
pmc: PMC7879834
doi:
Types de publication
Journal Article
Langues
eng
Pagination
303-309Informations de copyright
Copyright: © 2020 Journal of Human Reproductive Sciences.
Déclaration de conflit d'intérêts
There are no conflicts of interest.
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