Membrane potential regulates Hedgehog signalling in the Drosophila wing imaginal disc.
Drosophila
Hedgehog
membrane potential
signaling
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
07 04 2021
07 04 2021
Historique:
revised:
29
12
2020
received:
09
10
2020
accepted:
15
01
2021
pubmed:
26
2
2021
medline:
1
6
2021
entrez:
25
2
2021
Statut:
ppublish
Résumé
While the membrane potential of cells has been shown to be patterned in some tissues, specific roles for membrane potential in regulating signalling pathways that function during development are still being established. In the Drosophila wing imaginal disc, Hedgehog (Hh) from posterior cells activates a signalling pathway in anterior cells near the boundary which is necessary for boundary maintenance. Here, we show that membrane potential is patterned in the wing disc. Anterior cells near the boundary, where Hh signalling is most active, are more depolarized than posterior cells across the boundary. Elevated expression of the ENaC channel Ripped Pocket (Rpk), observed in these anterior cells, requires Hh. Antagonizing Rpk reduces depolarization and Hh signal transduction. Using genetic and optogenetic manipulations, in both the wing disc and the salivary gland, we show that membrane depolarization promotes membrane localization of Smoothened and augments Hh signalling, independently of Patched. Thus, membrane depolarization and Hh-dependent signalling mutually reinforce each other in cells immediately anterior to the compartment boundary.
Identifiants
pubmed: 33629503
doi: 10.15252/embr.202051861
pmc: PMC8024891
doi:
Substances chimiques
Drosophila Proteins
0
Hedgehog Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e51861Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM122490
Pays : United States
Organisme : NIH HHS
ID : R35GM122490
Pays : United States
Informations de copyright
© 2021 The Authors. Published under the terms of the CC BY 4.0 license.
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