Liver epigenome changes in patients with hepatopulmonary syndrome: A pilot study.
Adult
Apoptosis
/ genetics
Case-Control Studies
DNA Methylation
/ genetics
Epigenome
/ genetics
Epigenomics
/ methods
Female
Hepatopulmonary Syndrome
/ genetics
Humans
Liver
/ metabolism
Lung
/ pathology
Male
Middle Aged
Neovascularization, Physiologic
/ genetics
Neuroendocrine Cells
/ metabolism
Pilot Projects
Pulmonary Circulation
Pulmonary Gas Exchange
/ physiology
Severity of Illness Index
Vascular Remodeling
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
14
04
2020
accepted:
22
12
2020
entrez:
25
2
2021
pubmed:
26
2
2021
medline:
29
7
2021
Statut:
epublish
Résumé
The hepatopulmonary syndrome (HPS) is defined by the presence of pulmonary gas exchange abnormalities due to intrapulmonary vascular dilatations in patients with chronic liver disease. Changes in DNA methylation reflect the genomic variation. Since liver transplant (LT) reverts HPS we hypothesized that it may be associated with specific liver epigenetic changes. Thus, the aim of this study was to investigate the role of the liver epigenome in patients with HPS. We extracted DNA from paraffin embedded liver tissue samples from 10 patients with HPS and 10 age-, sex- and MELD (Model for End-stage Liver Disease)-matched controls. DNA methylation was determined using the 850K array (Illumina). Weighted Gene Co-expression Network Analysis (WGCNA) was used to identify modules related to defining physiologic characteristics of HPS. Only 12 out of the 20 liver biopsies (7 HPS and 5 controls) had sufficient quality to be analyzed. None of the 802,688 DNA probes analyzed in the case control comparison achieved a significant False Discovery Rate (FDR). WGCNA identified 5 co-methylated gene-modules associated to HPS markers, mainly related to nervous and neuroendocrine system, apoptotic processes, gut bacterial translocation, angiogenesis and vascular remodeling ontologies. To conclude, HPS is associated with nervous/neuroendocrine system and vascular remodeling related liver epigenetic changes.
Identifiants
pubmed: 33630849
doi: 10.1371/journal.pone.0245046
pii: PONE-D-20-10495
pmc: PMC7906328
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0245046Déclaration de conflit d'intérêts
The authors have delcared that no competing interests exists.
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