A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs.

Animals Antibodies, Neutralizing / immunology Antibodies, Viral / immunology Antigens, Viral / genetics COVID-19 / immunology COVID-19 Vaccines / genetics Disease Models, Animal Gene Expression Gene Order Genetic Vectors / genetics Immunophenotyping Lung / immunology Macaca Macrophages, Alveolar / immunology Mice SARS-CoV-2 / immunology Spike Glycoprotein, Coronavirus / genetics T-Lymphocyte Subsets / immunology Vaccination / methods Vaccines, DNA / genetics Vaccinia virus / genetics
CD8 T cells COVID-19 Modified Vaccinia Ankara Neutralizing antibody Protection Rhesus macaques SARS-CoV-2 Spike Vaccine

Journal

Immunity
ISSN: 1097-4180
Titre abrégé: Immunity
Pays: United States
ID NLM: 9432918

Informations de publication

Date de publication:
09 03 2021
Historique:
received: 23 10 2020
revised: 04 12 2020
accepted: 29 01 2021
pubmed: 26 2 2021
medline: 23 3 2021
entrez: 25 2 2021
Statut: ppublish

Résumé

A combination of vaccination approaches will likely be necessary to fully control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Here, we show that modified vaccinia Ankara (MVA) vectors expressing membrane-anchored pre-fusion stabilized spike (MVA/S) but not secreted S1 induced strong neutralizing antibody responses against SARS-CoV-2 in mice. In macaques, the MVA/S vaccination induced strong neutralizing antibodies and CD8

Identifiants

DOI: 10.1016/j.immuni.2021.02.001 PMID: 33631118 PMC: PMC7859620
pubmed: 33631118
pii: S1074-7613(21)00042-X
doi: 10.1016/j.immuni.2021.02.001
pmc: PMC7859620
pii:
doi:

Substances chimiques

Antibodies, Neutralizing 0
Antibodies, Viral 0
Antigens, Viral 0
COVID-19 Vaccines 0
Spike Glycoprotein, Coronavirus 0
Vaccines, DNA 0
spike protein, SARS-CoV-2 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

542-556.e9

Subventions

Organisme : NIAID NIH HHS
ID : HHSN272201400008C
Pays : United States
Organisme : NIH HHS
ID : P51 OD011132
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI148378
Pays : United States
Organisme : NIH HHS
ID : S10 OD026799
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests R.R.A., Sailaja Gangadhara, and N.K.R. are co-inventors of the MVA/S vaccine technology. Emory University filed a patent on this technology. R.R.A. serves as a SAB member for Heat Biologics Inc.

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Auteurs

Nanda Kishore Routhu (NK)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Narayanaiah Cheedarla (N)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Sailaja Gangadhara (S)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Venkata Satish Bollimpelli (VS)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Arun K Boddapati (AK)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Pathology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Ayalnesh Shiferaw (A)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Sheikh Abdul Rahman (SA)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Anusmita Sahoo (A)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Venkata Viswanadh Edara (VV)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Pediatrics, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA.

Lilin Lai (L)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Pediatrics, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA.

Katharine Floyd (K)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Pediatrics, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA.

Shelly Wang (S)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.

Stephanie Fischinger (S)

Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.

Caroline Atyeo (C)

Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.

Sally A Shin (SA)

Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.

Sanjeev Gumber (S)

Division of Pathology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.

Shannon Kirejczyk (S)

Division of Pathology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.

Joyce Cohen (J)

Division of Animal Resources, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.

Sherrie M Jean (SM)

Division of Animal Resources, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.

Jennifer S Wood (JS)

Division of Animal Resources, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.

Fawn Connor-Stroud (F)

Division of Animal Resources, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.

Rachelle L Stammen (RL)

Division of Animal Resources, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USA.

Amit A Upadhyay (AA)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.

Kathryn Pellegrini (K)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.

David Montefiori (D)

Department of Surgery, Duke University School of Medicine, Durham, NC 27710, USA.

Pei-Yong Shi (PY)

Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX, USA.

Vineet D Menachery (VD)

Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX, USA.

Galit Alter (G)

Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.

Thomas H Vanderford (TH)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA.

Steven E Bosinger (SE)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Pathology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA.

Mehul S Suthar (MS)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Pediatrics, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA.

Rama Rao Amara (RR)

Emory Vaccine Center, Division of Microbiology and Immunology, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA. Electronic address: ramara@emory.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps neutralisants › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antiviraux › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes viraux › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins antiviraux › Vaccins contre la COVID-19 › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Phénomènes génétiques › Ordre des gènes › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Vecteurs génétiques › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Immunophénotypage › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Appareil respiratoire › Poumon › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Macrophages alvéolaires › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › SARS-CoV-2 › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines de l'enveloppe virale › Protéines de fusion virale › Glycoprotéine de spicule des coronavirus › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › Sous-populations de lymphocytes T › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Prévention primaire › Immunisation › Vaccination › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Vaccins synthétiques › Vaccins à base d'acide nucléique › Vaccins à ADN › A modified vaccinia Ankara vector-based...
questionsmedicales.fr › Virus › Virus à ADN › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Virus de la vaccine › A modified vaccinia Ankara vector-based...