Selective Release of Recombinant Periplasmic Protein From

continuous downstream processing electroporation host cell impurities outer membrane periplasmic protein primary recovery pulsed electric field selective product release

Journal

Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513

Informations de publication

Date de publication:
2020
Historique:
received: 24 07 2020
accepted: 29 12 2020
entrez: 26 2 2021
pubmed: 27 2 2021
medline: 27 2 2021
Statut: epublish

Résumé

To date, high-pressure homogenization is the standard method for cell disintegration before the extraction of cytosolic and periplasmic protein from

Identifiants

pubmed: 33634078
doi: 10.3389/fbioe.2020.586833
pmc: PMC7900513
doi:

Types de publication

Journal Article

Langues

eng

Pagination

586833

Informations de copyright

Copyright © 2021 Schottroff, Kastenhofer, Spadiut, Jaeger and Wurm.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Felix Schottroff (F)

Institute of Food Technology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
BOKU Core Facility Food & Bio Processing, Vienna, Austria.

Jens Kastenhofer (J)

Research Division Biochemical Engineering, Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria.

Oliver Spadiut (O)

Research Division Biochemical Engineering, Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria.

Henry Jaeger (H)

Institute of Food Technology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.

David J Wurm (DJ)

Research Division Biochemical Engineering, Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria.

Classifications MeSH