Zonula occludens-1 demonstrates a unique appearance in buccal mucosa over several layers.
Barrier function
Buccal mucosa
Claudin-1
Distribution
Tight junction
Zonula occludens-1
Journal
Cell and tissue research
ISSN: 1432-0878
Titre abrégé: Cell Tissue Res
Pays: Germany
ID NLM: 0417625
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
21
11
2019
accepted:
24
01
2021
pubmed:
27
2
2021
medline:
15
12
2021
entrez:
26
2
2021
Statut:
ppublish
Résumé
Tight junctions (TJs) firmly seal epithelial cells and are key players in the epithelial barrier. TJs consist of several proteins, including those of the transmembrane claudin family and the scaffold zonula occludens (ZO) family. Epithelial tissues are exposed to different conditions: to air in the stratified epithelium of the skin and to liquids in the monolayer of the intestine. The TJs in stratified oral mucosal epithelium have remained insufficiently elucidated in terms of distributions, appearances and barrier functions of TJ proteins in normal buccal mucosa. We investigated these and ZO-1 and claudin-1 were found to be expressed in the top third and in the bottom three quarters of the mucosal epithelium. ZO-1 in the buccal mucosa was found to have an irregular linear appearance. ZO-1 in the buccal mucosa continuously existed in several layers. Electron microscopy revealed the buccal mucosa to have kissing points. In a biotin permeation assay that sought to investigate inside-outside barrier function, the biotin tracer penetrated several ZO-1 layers but did not pass through all the ZO-1 layers. We found that the oral mucosal cell knockdown of TJP1 or CLDN1 resulted in decreases of TER but no significant change in FITC-dextran leakage. Our results suggest that the distribution and appearance of ZO-1 in the buccal mucosa differ from those in the skin. We were unable to prove barrier function in this study but we did show barrier function against small molecules in vivo and against ions in vitro.
Identifiants
pubmed: 33635425
doi: 10.1007/s00441-021-03425-8
pii: 10.1007/s00441-021-03425-8
doi:
Substances chimiques
CLDN1 protein, human
0
Claudin-1
0
TJP1 protein, human
0
Zonula Occludens-1 Protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
691-702Références
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