High stroma-derived WNT5A is an indicator for low-risk prostate cancer.


Journal

FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716

Informations de publication

Date de publication:
04 2021
Historique:
received: 01 02 2021
accepted: 25 02 2021
pubmed: 28 2 2021
medline: 7 1 2022
entrez: 27 2 2021
Statut: ppublish

Résumé

Prostate cancer (PCa) is a major cause of cancer-related death in men. Tumor-derived protein derived from Wnt5A gene (WNT5A) plays an important role in primary and metastatic PCa. Surrounding stroma cells also produce WNT5A, which may modulate the biology of PCa. Here, we assessed the role of stroma-derived WNT5A (stWNT5A) in primary PCa. A tissue microarray of samples obtained from 400 patients who underwent radical prostatectomy and control samples from 41 patients with benign prostate hyperplasia (BPH) was immunohistochemically assessed for expression of stWNT5A. The cores were scored for staining intensity: 0 (no staining), 1 (weak), 2 (moderate), or 3 (strong) and the stained stromal surface area: 0 (0%), 1 (1-25%), 2 (26-50%), 3 (51-75%), or 4 (76-100%). Gleason Score (GS) and TNM-stage were assessed by stratifying the cohort into high-risk (≥ pT3, pN1, GS ≥ 8) and non-high-risk patients. Ki67 and TUNEL assays were performed to assess proliferation and apoptosis. Expression of stWNT5A in BPH and tumor-free control samples was 1.2-fold higher compared to tumor samples (P < 0.001). Non-high-risk patients had a higher stWNT5A score than high-risk patients (P < 0.05). stWNT5A expression was not correlated with overall and cancer-specific survival. Proliferation (r

Identifiants

pubmed: 33639039
doi: 10.1002/2211-5463.13131
pmc: PMC8016115
doi:

Substances chimiques

Biomarkers, Tumor 0
WNT5A protein, human 0
Wnt-5a Protein 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1186-1194

Informations de copyright

© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

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Auteurs

Wadim Kisel (W)

University Center for Traumatology, Orthopedics and Plastic Surgery, Technische Universität Dresden, Germany.

Stefanie Conrad (S)

Division of Endocrinology, Diabetes, and Bone Diseases, Department of Medicine III and University Center for Healthy Aging, Technische Universität Dresden, Germany.

Angelika Borkowetz (A)

Department of Urology, Technische Universität Dresden, Germany.

Giulia Furesi (G)

Division of Endocrinology, Diabetes, and Bone Diseases, Department of Medicine III and University Center for Healthy Aging, Technische Universität Dresden, Germany.

Susanne Füssel (S)

Department of Urology, Technische Universität Dresden, Germany.

Ulrich Sommer (U)

Department of Pathology, Technische Universität Dresden, Germany.

Martina Rauner (M)

Division of Endocrinology, Diabetes, and Bone Diseases, Department of Medicine III and University Center for Healthy Aging, Technische Universität Dresden, Germany.

Christian Thomas (C)

Department of Urology, Technische Universität Dresden, Germany.

Gustavo B Baretton (GB)

Department of Pathology, Technische Universität Dresden, Germany.

Klaus-Dieter Schaser (KD)

University Center for Traumatology, Orthopedics and Plastic Surgery, Technische Universität Dresden, Germany.

Christine Hofbauer (C)

University Cancer Center, Technische Universität Dresden, Germany.

Lorenz C Hofbauer (LC)

Division of Endocrinology, Diabetes, and Bone Diseases, Department of Medicine III and University Center for Healthy Aging, Technische Universität Dresden, Germany.

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Classifications MeSH