Expression of endoplasmic reticulum stress mRNAs in otitis media.
Adolescent
Adult
Aged
Aged, 80 and over
Audiometry, Pure-Tone
Cholesteatoma, Middle Ear
/ metabolism
Chronic Disease
Endoplasmic Reticulum Stress
/ genetics
Female
Humans
Male
Middle Aged
Otitis Media
/ genetics
Otitis Media with Effusion
/ metabolism
RNA, Messenger
/ metabolism
Real-Time Polymerase Chain Reaction
Young Adult
Chronic otitis media, Cholesteatoma
Endoplasmic reticulum stress
Journal
Acta oto-laryngologica
ISSN: 1651-2251
Titre abrégé: Acta Otolaryngol
Pays: England
ID NLM: 0370354
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
pubmed:
2
3
2021
medline:
20
11
2021
entrez:
1
3
2021
Statut:
ppublish
Résumé
Endoplasmic reticulum (ER) stress is a cellular defense mechanism that occurs when ER function is impaired. This study was designed to evaluate the expression of major mRNAs of ER stress in patients with otitis media with effusion (OME), chronic otitis media (COM), and COM with cholesteatoma (CholeOM). Specimens were collected during surgery from patients with OME, COM, and CholeOM, and the levels of ER stress mRNAs measured by real-time polymerase chain reaction. Levels of ER stress mRNAs were compared in the three groups and correlated with clinical findings and pus culture results. The level of CHOP mRNA was higher, and the levels of sXBP1 and ATF6 mRNAs lower, in the OME than in the other two groups ( ER stress may be involved in the pathophysiology of OM and the levels of ER stress mRNAs were expressed differently in each type of otitis media according to bacterial culture test results.
Sections du résumé
BACKGROUND
BACKGROUND
Endoplasmic reticulum (ER) stress is a cellular defense mechanism that occurs when ER function is impaired.
OBJECTIVE
OBJECTIVE
This study was designed to evaluate the expression of major mRNAs of ER stress in patients with otitis media with effusion (OME), chronic otitis media (COM), and COM with cholesteatoma (CholeOM).
MATERIAL AND METHODS
METHODS
Specimens were collected during surgery from patients with OME, COM, and CholeOM, and the levels of ER stress mRNAs measured by real-time polymerase chain reaction. Levels of ER stress mRNAs were compared in the three groups and correlated with clinical findings and pus culture results.
RESULTS
RESULTS
The level of CHOP mRNA was higher, and the levels of sXBP1 and ATF6 mRNAs lower, in the OME than in the other two groups (
CONCLUSIONS AND SIGNIFICANCE
CONCLUSIONS
ER stress may be involved in the pathophysiology of OM and the levels of ER stress mRNAs were expressed differently in each type of otitis media according to bacterial culture test results.
Identifiants
pubmed: 33641571
doi: 10.1080/00016489.2021.1883733
doi:
Substances chimiques
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM