Randomly amplified polymorphic DNA analysis of

Staphylococcus chromogenes mastitis methicillin resistance non-aureus staphylococci randomly amplified polymorphic DNA

Journal

Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872

Informations de publication

Date de publication:
Jan 2021
Historique:
received: 13 07 2020
accepted: 14 12 2020
entrez: 1 3 2021
pubmed: 2 3 2021
medline: 2 3 2021
Statut: ppublish

Résumé

In recent times, non-aureus staphylococci (NAS) have emerged as the major organisms isolated from mastitis cases in dairy animals, with a predominance of Thirty-seven The amplified DNA fragments ranged from 150 to 3000 base pairs and yielded several RAPD profiles. Further analysis using Digital Image Correlation Engine correlation coefficient and UPGMA method showed that the 37 isolates could be classified into 12 distinct RAPD types (A to L) at 62% similarity ( In the absence of defined multilocus sequence type protocols or sufficient sequences available in the public domain, RAPD can be employed to determine genetic diversity of

Sections du résumé

BACKGROUND AND AIM OBJECTIVE
In recent times, non-aureus staphylococci (NAS) have emerged as the major organisms isolated from mastitis cases in dairy animals, with a predominance of
MATERIALS AND METHODS METHODS
Thirty-seven
RESULTS RESULTS
The amplified DNA fragments ranged from 150 to 3000 base pairs and yielded several RAPD profiles. Further analysis using Digital Image Correlation Engine correlation coefficient and UPGMA method showed that the 37 isolates could be classified into 12 distinct RAPD types (A to L) at 62% similarity (
CONCLUSION CONCLUSIONS
In the absence of defined multilocus sequence type protocols or sufficient sequences available in the public domain, RAPD can be employed to determine genetic diversity of

Identifiants

pubmed: 33642816
doi: 10.14202/vetworld.2021.285-291
pii: Vetworld-14-285
pmc: PMC7896888
doi:

Types de publication

Journal Article

Langues

eng

Pagination

285-291

Informations de copyright

Copyright: © Sheela, et al.

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Auteurs

P Sheela (P)

Department of Veterinary Microbiology, Veterinary College, Shivamogga, Karnataka Veterinary Animal and Fisheries Sciences University, Shivamogga, Karnataka, India.

Malathi Shekar (M)

Bioinformatics Centre, Department of Aquatic Animal Health Management, College of Fisheries, Mangaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Mangaluru, Karnataka, India.

Shrikrishna Isloor (S)

Department of Microbiology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.

D Rathnamma (D)

Department of Microbiology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.

B M Veeregowda (BM)

Department of Microbiology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.

M L Satyanarayana (ML)

Department of Pathology, Veterinary College, Bengaluru, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India.

S Sundareshan (S)

Department of Veterinary Microbiology, Veterinary College, Shivamogga, Karnataka Veterinary Animal and Fisheries Sciences University, Shivamogga, Karnataka, India.

B E Shambulingappa (BE)

Department of Veterinary Microbiology, Veterinary College, Shivamogga, Karnataka Veterinary Animal and Fisheries Sciences University, Shivamogga, Karnataka, India.

Nagendra R Hegde (NR)

National Institute of Animal Biotechnology, Gachibowli, Hyderabad, Telangana, India.

Classifications MeSH