Lipid droplets in mammalian eggs are utilized during embryonic diapause.
blastocyst
embryonic diapause
lipid droplets
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
09 03 2021
09 03 2021
Historique:
entrez:
2
3
2021
pubmed:
3
3
2021
medline:
18
8
2021
Statut:
ppublish
Résumé
Embryonic diapause (ED) is a temporary arrest of an embryo at the blastocyst stage when it waits for the uterine receptivity signal to implant. ED used by over 100 species may also occur in normally "nondiapausing" mammals when the uterine receptivity signal is blocked or delayed. A large number of lipid droplets (LDs) are stored throughout the preimplantation embryo development, but the amount of lipids varies greatly across different mammalian species. Yet, the role of LDs in the mammalian egg and embryo remains unknown. Here, using a mouse model, we provide evidence that LDs play a crucial role in maintaining ED. By mechanical removal of LDs from zygotes, we demonstrated that delipidated embryos are unable to survive during ED. LDs are not essential for normal prompt implantation, without ED. We further demonstrated that with the progression of ED, the amount of intracellular lipid reduces, and composition changes. This decrease in lipid is caused by a switch from carbohydrate metabolism to lipid catabolism in diapausing blastocysts, which also exhibit increased release of exosomes reflecting elevated embryonic signaling to the mother. We have also shown that presence of LDs in the oocytes of various mammals positively corelates with their species-specific length of diapause. Our results reveal the functional role of LDs in embryonic development. These results can help to develop diagnostic techniques and treatment of recurrent implantation failure and will likely ignite further studies in developmental biology and reproductive medicine fields.
Identifiants
pubmed: 33649221
pii: 2018362118
doi: 10.1073/pnas.2018362118
pmc: PMC7958255
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
The authors declare no competing interest.
Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
J Reprod Fertil. 1996 Mar;106(2):299-306
pubmed: 8699414
J Infect Dis Med. 2017 Sep;2(2):
pubmed: 29034376
Biol Reprod. 2011 Jul;85(1):62-9
pubmed: 21311036
Genome Biol. 2014;15(12):550
pubmed: 25516281
Biol Reprod. 1979 Sep;21(2):517-21
pubmed: 486673
Cell. 2015 Apr 9;161(2):291-306
pubmed: 25860611
PLoS One. 2012;7(3):e33027
pubmed: 22427933
Reproduction. 2003 Sep;126(3):299-308
pubmed: 12968937
Nat Cell Biol. 2015 Jun;17(6):759-70
pubmed: 25961502
Reproduction. 2012 Apr;143(4):417-27
pubmed: 22408180
J Extracell Vesicles. 2013 May 27;2:
pubmed: 24009894
Differentiation. 2006 Feb;74(1):11-8
pubmed: 16466396
Histochem J. 1982 Sep;14(5):823-35
pubmed: 6182131
Development. 2018 Feb 23;145(4):
pubmed: 29475974
J Exp Zool. 1968 May;168(1):49-59
pubmed: 5700166
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W193-200
pubmed: 17478515
Science. 2002 Jun 21;296(5576):2185-8
pubmed: 12077405
Biol Reprod. 2014 Mar 13;90(3):52
pubmed: 24451987
Curr Opin Cell Biol. 2015 Aug;35:91-7
pubmed: 25988547
Development. 2016 Jun 15;143(12):2238-47
pubmed: 27151947
Nature. 1977 Jan 6;265(5589):53-5
pubmed: 556803
Proc Natl Acad Sci U S A. 2004 Jul 13;101(28):10326-31
pubmed: 15232000
Nature. 1995 Mar 30;374(6521):416
pubmed: 7700349
Proc Natl Acad Sci U S A. 2019 Aug 13;116(33):16621-16630
pubmed: 31346081
Development. 2017 Sep 15;144(18):3199-3210
pubmed: 28928280
J Endocrinol. 1968 Nov;42(3):453-63
pubmed: 4179775
J Reprod Fertil. 1966 Oct;12(2):391-4
pubmed: 5951130
Nat Rev Mol Cell Biol. 2018 Apr;19(4):213-228
pubmed: 29339798
Biol Rev Camb Philos Soc. 2014 Nov;89(4):889-912
pubmed: 24517909
J Reprod Fertil. 1981 Mar;61(2):307-15
pubmed: 7205777
Trends Endocrinol Metab. 2016 Oct;27(10):696-705
pubmed: 27365163
Hum Reprod. 1999 Oct;14(10):2434-6
pubmed: 10527963
Birth Defects Res B Dev Reprod Toxicol. 2013 Apr;98(2):200-5
pubmed: 23526690
Dev Cell. 2015 Mar 23;32(6):678-92
pubmed: 25752962
Biol Reprod. 1987 Nov;37(4):775-8
pubmed: 3689846
Bioinformatics. 2009 May 1;25(9):1105-11
pubmed: 19289445
J Reprod Fertil. 1984 Sep;72(1):9-13
pubmed: 6540809
Lab Anim Sci. 1985 Apr;35(2):142-5
pubmed: 4039779
J Reprod Fertil. 1990 Jan;88(1):361-8
pubmed: 2313649
Semin Cell Dev Biol. 2014 Oct;34:56-64
pubmed: 24862857
R Soc Open Sci. 2017 Apr 26;4(4):161098
pubmed: 28484627
BMC Genomics. 2017 Aug 7;18(1):583
pubmed: 28784092
J Hyg (Lond). 1941 Sep;41(2):131-8
pubmed: 20475584