The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D.
Animals
Biomarkers
/ metabolism
Cell Culture Techniques
/ methods
Cell Differentiation
Cell Lineage
Cells, Cultured
Endoderm
/ cytology
Human Embryonic Stem Cells
/ cytology
Humans
Imaging, Three-Dimensional
Lung
/ cytology
Mice
Parainfluenza Virus 3, Human
/ physiology
Pluripotent Stem Cells
/ cytology
Journal
Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
25
03
2019
accepted:
26
11
2020
pubmed:
3
3
2021
medline:
5
5
2021
entrez:
2
3
2021
Statut:
ppublish
Résumé
Lung and airway epithelial cells generated in vitro from human pluripotent stem cells (hPSCs) have applications in regenerative medicine, modeling of lung disease, drug screening and studies of human lung development. Here, we describe a strategy for directed differentiation of hPSCs into mature lung and airway epithelial cells obtained through maturation of NKX2.1
Identifiants
pubmed: 33649566
doi: 10.1038/s41596-020-00476-z
pii: 10.1038/s41596-020-00476-z
pmc: PMC9460941
mid: NIHMS1780599
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1802-1829Subventions
Organisme : NCRR NIH HHS
ID : S10 RR027050
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI031971
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI114736
Pays : United States
Organisme : NIH HHS
ID : S10 OD020056
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL134760
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL120046
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI031971
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL142727
Pays : United States
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