Persistent SARS-CoV-2 RNA Shedding Without Evidence of Infectiousness: A Cohort Study of Individuals With COVID-19.
Adolescent
Adult
Age Factors
Aged
Aged, 80 and over
COVID-19
/ diagnosis
COVID-19 Nucleic Acid Testing
Child
Child, Preschool
Contact Tracing
Female
Humans
Male
Middle Aged
Nasopharynx
/ pathology
RNA, Viral
/ isolation & purification
SARS-CoV-2
/ genetics
Time Factors
Virus Replication
Virus Shedding
Young Adult
COVID-19
SARS-CoV-2
infectious period
infectivity
viral culture
viral shedding
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
28 Oct 2021
28 Oct 2021
Historique:
received:
16
10
2020
accepted:
17
02
2021
pubmed:
3
3
2021
medline:
9
11
2021
entrez:
2
3
2021
Statut:
ppublish
Résumé
To better understand severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding and infectivity, we estimated SARS-CoV-2 RNA shedding duration, described participant characteristics associated with the first negative rRT-PCR test (resolution), and determined if replication-competent viruses was recoverable ≥10 days after symptom onset. We collected serial nasopharyngeal specimens from 109 individuals with rRT-PCR-confirmed COVID-19 in Utah and Wisconsin. We calculated viral RNA shedding resolution probability using the Kaplan-Meier estimator and evaluated characteristics associated with shedding resolution using Cox proportional hazards regression. We attempted viral culture for 35 rRT-PCR-positive nasopharyngeal specimens collected ≥10 days after symptom onset. The likelihood of viral RNA shedding resolution at 10 days after symptom onset was approximately 3%. Time to shedding resolution was shorter among participants aged <18 years (adjusted hazards ratio [aHR], 3.01; 95% confidence interval [CI], 1.6-5.6) and longer among those aged ≥50 years (aHR, 0.50; 95% CI, .3-.9) compared to participants aged 18-49 years. No replication-competent viruses were recovered. Although most patients were positive for SARS-CoV-2 for ≥10 days after symptom onset, our findings suggest that individuals with mild to moderate COVID-19 are unlikely to be infectious ≥10 days after symptom onset.
Sections du résumé
BACKGROUND
BACKGROUND
To better understand severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding and infectivity, we estimated SARS-CoV-2 RNA shedding duration, described participant characteristics associated with the first negative rRT-PCR test (resolution), and determined if replication-competent viruses was recoverable ≥10 days after symptom onset.
METHODS
METHODS
We collected serial nasopharyngeal specimens from 109 individuals with rRT-PCR-confirmed COVID-19 in Utah and Wisconsin. We calculated viral RNA shedding resolution probability using the Kaplan-Meier estimator and evaluated characteristics associated with shedding resolution using Cox proportional hazards regression. We attempted viral culture for 35 rRT-PCR-positive nasopharyngeal specimens collected ≥10 days after symptom onset.
RESULTS
RESULTS
The likelihood of viral RNA shedding resolution at 10 days after symptom onset was approximately 3%. Time to shedding resolution was shorter among participants aged <18 years (adjusted hazards ratio [aHR], 3.01; 95% confidence interval [CI], 1.6-5.6) and longer among those aged ≥50 years (aHR, 0.50; 95% CI, .3-.9) compared to participants aged 18-49 years. No replication-competent viruses were recovered.
CONCLUSIONS
CONCLUSIONS
Although most patients were positive for SARS-CoV-2 for ≥10 days after symptom onset, our findings suggest that individuals with mild to moderate COVID-19 are unlikely to be infectious ≥10 days after symptom onset.
Identifiants
pubmed: 33649773
pii: 6154064
doi: 10.1093/infdis/jiab107
pmc: PMC7989388
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1362-1371Investigateurs
Lindsey M Duca
(LM)
Elizabeth Rabold
(E)
Radhika Gharpure
(R)
Henry Njuguna
(H)
Patrick Dawson
(P)
Erin E Conners
(EE)
Victoria Fields
(V)
Phillip Salvatore
(P)
Perrine Marcenac
(P)
Hannah E Reses
(HE)
Mark Fajans
(M)
Rebecca L Laws
(RL)
Sherry Yin
(S)
Dongni Ye
(D)
Eric Pevzner
(E)
Katherine Battey
(K)
Cuc Tran
(C)
Michelle O'Hegarty
(M)
Jeni Vuong
(J)
Rebecca J Chancey
(RJ)
Christopher J Gregory
(CJ)
Michelle Banks
(M)
Jared Rispens
(J)
Sandra Lester
(S)
Lisa Mills
(L)
Alicia Fry
(A)
Scott Nabity
(S)
Brandi Freeman
(B)
Sean Buono
(S)
Informations de copyright
Published by Oxford University Press for the Infectious Diseases Society of America 2021.