Model for Murine Gut Colonization by Bifidobacteria.
Antibiotic treatment
Bifidobacterial colonization
Gavage
Microbiota depletion
Murine model
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
2
3
2021
pubmed:
3
3
2021
medline:
10
4
2021
Statut:
ppublish
Résumé
Members of the Bifidobacterium genus are some of the earliest and most important colonizers of the human neonatal gastrointestinal tract (GIT), exerting wide-ranging effects on early development of the host. However, human isolates of bifidobacteria are very inefficient colonizers of specific-pathogen-free (SPF) mice creating a technical barrier to discovery and applied research in this area. We have developed a reproducible model to facilitate transient colonization of SPF mice with human isolates of this genus through prior depletion of the gut resident microbiota with antibiotics. This chapter outlines the technical details for performing efficient microbiota depletion with antibiotics and subsequent administration of bifidobacteria for colonization.
Identifiants
pubmed: 33649953
doi: 10.1007/978-1-0716-1274-3_11
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
131-139Références
Gonzalez-Rodriguez I, Ruiz L, Gueimonde M, Margolles A, Sanchez B (2013) Factors involved in the colonization and survival of bifidobacteria in the gastrointestinal tract. FEMS Microbiol Lett 340(1):1–10
doi: 10.1111/1574-6968.12056
Leahy SC, Higgins DG, Fitzgerald GF, van Sinderen D (2005) Getting better with bifidobacteria. J Appl Microbiol 98(6):1303–1315
doi: 10.1111/j.1365-2672.2005.02600.x
Jeon SG, Kayama H, Ueda Y, Takahashi T, Asahara T, Tsuji H et al (2012) Probiotic Bifidobacterium breve induces IL-10-producing Tr1 cells in the colon. PLoS Pathog 8(5):e1002714
doi: 10.1371/journal.ppat.1002714
Veiga P, Gallini CA, Beal C, Michaud M, Delaney ML, DuBois A et al (2010) Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes. Proc Natl Acad Sci U S A 107(42):18132–18137
doi: 10.1073/pnas.1011737107
Maldonado-Gomez MX, Martinez I, Bottacini F, O'Callaghan A, Ventura M, van Sinderen D et al (2016) Stable engraftment of Bifidobacterium longum AH1206 in the human gut depends on individualized features of the resident microbiome. Cell Host Microbe 20(4):515–526
doi: 10.1016/j.chom.2016.09.001
Fujiwara S, Seto Y, Kimura A, Hashiba H (2001) Intestinal transit of an orally administered streptomycin-rifampicin-resistant variant of Bifidobacterium longum SBT2928: its long-term survival and effect on the intestinal microflora and metabolism. J Appl Microbiol 90(1):43–52
doi: 10.1046/j.1365-2672.2001.01205.x
Grimm V, Radulovic K, Riedel CU (2015) Colonization of C57BL/6 mice by a potential probiotic Bifidobacterium bifidum strain under germ-free and specific pathogen-free conditions and during experimental colitis. PLoS One 10(10):e0139935
doi: 10.1371/journal.pone.0139935
Nigro G, Rossi R, Commere PH, Jay P, Sansonetti PJ (2014) The cytosolic bacterial peptidoglycan sensor Nod2 affords stem cell protection and links microbes to gut epithelial regeneration. Cell Host Microbe 15(6):792–798
doi: 10.1016/j.chom.2014.05.003
Reikvam DH, Erofeev A, Sandvik A, Grcic V, Jahnsen FL, Gaustad P et al (2011) Depletion of murine intestinal microbiota: effects on gut mucosa and epithelial gene expression. PLoS One 6(3):e17996
doi: 10.1371/journal.pone.0017996
Ceylani T, Jakubowska-Dogru E, Gurbanov R, Teker HT, Gozen AG (2018) The effects of repeated antibiotic administration to juvenile BALB/c mice on the microbiota status and animal behavior at the adult age. Heliyon 4(6):e00644
doi: 10.1016/j.heliyon.2018.e00644
Turroni F, Foroni E, Pizzetti P, Giubellini V, Ribbera A, Merusi P et al (2009) Exploring the diversity of the bifidobacterial population in the human intestinal tract. Appl Environ Microbiol 75(6):1534–1545
doi: 10.1128/AEM.02216-08