Association of Toll-like receptor 7 variants with life-threatening COVID-19 disease in males: findings from a nested case-control study.
COVID-19
LASSO Logistic Regression Analysis
TLR7
genetics
genomics
human
medicine
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
02 03 2021
02 03 2021
Historique:
received:
16
02
2021
accepted:
24
02
2021
pubmed:
3
3
2021
medline:
7
4
2021
entrez:
2
3
2021
Statut:
epublish
Résumé
Recently, loss-of-function variants in TLR7 were identified in two families in which COVID-19 segregates like an X-linked recessive disorder environmentally conditioned by SARS-CoV-2. We investigated whether the two families represent the tip of the iceberg of a subset of COVID-19 male patients. This is a nested case-control study in which we compared male participants with extreme phenotype selected from the Italian GEN-COVID cohort of SARS-CoV-2-infected participants (<60 y, 79 severe cases versus 77 control cases). We applied the LASSO Logistic Regression analysis, considering only rare variants on young male subsets with extreme phenotype, picking up TLR7 as the most important susceptibility gene. Overall, we found TLR7 deleterious variants in 2.1% of severely affected males and in none of the asymptomatic participants. The functional gene expression profile analysis demonstrated a reduction in TLR7-related gene expression in patients compared with controls demonstrating an impairment in type I and II IFN responses. Young males with TLR7 loss-of-function variants and severe COVID-19 represent a subset of male patients contributing to disease susceptibility in up to 2% of severe COVID-19. Funded by private donors for the Host Genetics Research Project, the Intesa San Paolo for 2020 charity fund, and the Host Genetics Initiative. NCT04549831.
Sections du résumé
Background
Recently, loss-of-function variants in TLR7 were identified in two families in which COVID-19 segregates like an X-linked recessive disorder environmentally conditioned by SARS-CoV-2. We investigated whether the two families represent the tip of the iceberg of a subset of COVID-19 male patients.
Methods
This is a nested case-control study in which we compared male participants with extreme phenotype selected from the Italian GEN-COVID cohort of SARS-CoV-2-infected participants (<60 y, 79 severe cases versus 77 control cases). We applied the LASSO Logistic Regression analysis, considering only rare variants on young male subsets with extreme phenotype, picking up TLR7 as the most important susceptibility gene.
Results
Overall, we found TLR7 deleterious variants in 2.1% of severely affected males and in none of the asymptomatic participants. The functional gene expression profile analysis demonstrated a reduction in TLR7-related gene expression in patients compared with controls demonstrating an impairment in type I and II IFN responses.
Conclusions
Young males with TLR7 loss-of-function variants and severe COVID-19 represent a subset of male patients contributing to disease susceptibility in up to 2% of severe COVID-19.
Funding
Funded by private donors for the Host Genetics Research Project, the Intesa San Paolo for 2020 charity fund, and the Host Genetics Initiative.
Clinical trial number
NCT04549831.
Identifiants
pubmed: 33650967
doi: 10.7554/eLife.67569
pii: 67569
pmc: PMC7987337
doi:
pii:
Substances chimiques
TLR7 protein, human
0
Toll-Like Receptor 7
0
Banques de données
ClinicalTrials.gov
['NCT04549831']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Private Donors for Host Genetics Research Project
ID : D.L. n 18 of March 17
Organisme : Intesa San Paolo for 2020 charity fund
ID : N.B.2020/0119
Organisme : Host Genetics Initiative
ID : Dipartimenti di Eccellenza 2018-2020
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : Dipartimenti di Eccellenza 2018-2020
Organisme : Regione Toscana
ID : Bando Ricerca COVID-19 Toscana
Investigateurs
Floriana Valentino
(F)
Gabriella Doddato
(G)
Annarita Giliberti
(A)
Rossella Tita
(R)
Sara Amitrano
(S)
Mirella Bruttini
(M)
Susanna Croci
(S)
Ilaria Meloni
(I)
Maria Antonietta Mencarelli
(MA)
Caterina Lo Rizzo
(C)
Anna Maria Pinto
(AM)
Laura Di Sarno
(L)
Giada Beligni
(G)
Andrea Tommasi
(A)
Nicola Iuso
(N)
Francesca Montagnani
(F)
Massimiliano Fabbiani
(M)
Barbara Rossetti
(B)
Giacomo Zanelli
(G)
Elena Bargagli
(E)
Laura Bergantini
(L)
Miriana D'Alessandro
(M)
Paolo Cameli
(P)
David Bennett
(D)
Federico Anedda
(F)
Simona Marcantonio
(S)
Sabino Scolletta
(S)
Federico Franchi
(F)
Maria Antonietta Mazzei
(MA)
Susanna Guerrini
(S)
Edoardo Conticini
(E)
Luca Cantarini
(L)
Bruno Frediani
(B)
Danilo Tacconi
(D)
Chiara Spertilli
(C)
Marco Feri
(M)
Alice Donati
(A)
Raffaele Scala
(R)
Luca Guidelli
(L)
Genni Spargi
(G)
Marta Corridi
(M)
Cesira Nencioni
(C)
Leonardo Croci
(L)
Gian Piero Caldarelli
(GP)
Maurizio Spagnesi
(M)
Davide Romani
(D)
Paolo Piacentini
(P)
Elena Desanctis
(E)
Silvia Cappelli
(S)
Anna Canaccini
(A)
Agnese Verzuri
(A)
Valentina Anemoli
(V)
Agostino Ognibene
(A)
Antonella D'Arminio Monforte
(AD)
Federica Gaia Miraglia
(FG)
Massimo Girardis
(M)
Sophie Venturelli
(S)
Stefano Busani
(S)
Andrea Cossarizza
(A)
Andrea Antinori
(A)
Alessandra Vergori
(A)
Arianna Emiliozzi
(A)
Arianna Gabrieli
(A)
Agostino Riva
(A)
Pier Giorgio Scotton
(PG)
Francesca Andretta
(F)
Sandro Panese
(S)
Renzo Scaggiante
(R)
Francesca Gatti
(F)
Saverio Giuseppe Parisi
(SG)
Stefano Baratti
(S)
Melania Degli Antoni
(MD)
Matteo Della Monica
(MD)
Carmelo Piscopo
(C)
Mario Capasso
(M)
Roberta Russo
(R)
Immacolata Andolfo
(I)
Achille Iolascon
(A)
Giuseppe Fiorentino
(G)
Massimo Carella
(M)
Marco Castori
(M)
Giuseppe Merla
(G)
Gabriella Maria Squeo
(GM)
Filippo Aucella
(F)
Pamela Raggi
(P)
Carmen Marciano
(C)
Rita Perna
(R)
Matteo Bassetti
(M)
Antonio Di Biagio
(A)
Maurizio Sanguinetti
(M)
Luca Masucci
(L)
Serafina Valente
(S)
Marco Mandalà
(M)
Alessia Giorli
(A)
Lorenzo Salerni
(L)
Patrizia Zucchi
(P)
Pierpaolo Parravicini
(P)
Elisabetta Menatti
(E)
Tullio Trotta
(T)
Ferdinando Giannattasio
(F)
Gabriella Coiro
(G)
Fabio Lena
(F)
Domenico A Coviello
(DA)
Cristina Mussini
(C)
Giancarlo Bosio
(G)
Enrico Martinelli
(E)
Sandro Mancarella
(S)
Luisa Tavecchia
(L)
Marco Gori
(M)
Lia Crotti
(L)
Gianfranco Parati
(G)
Chiara Gabbi
(C)
Isabella Zanella
(I)
Marco Rizzi
(M)
Franco Maggiolo
(F)
Diego Ripamonti
(D)
Tiziana Bachetti
(T)
Maria Teresa La Rovere
(MT)
Simona Sarzi-Braga
(S)
Maurizio Bussotti
(M)
Mario Chiariello
(M)
Mary Ann Belli
(MA)
Simona Dei
(S)
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021, Fallerini et al.
Déclaration de conflit d'intérêts
CF, SD, SM, EB, NP, DF, FP, ES, MB, FF, MP, SL, FC, EQ, MV, SR, MS, MB, OS, KC, SF, FM, AR, MM, EF No competing interests declared
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