Urinary Extracellular Vesicles: Ultracentrifugation Method.
Exosome
Isolation
PS affinity method
Sucrose–D2O cushion
Tamm–Horsfall protein
Tim4
Ultracentrifugation
Urinary extracellular vesicles
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
2
3
2021
pubmed:
3
3
2021
medline:
7
4
2021
Statut:
ppublish
Résumé
Recently, urinary extracellular vesicles (EVs) have garnered interest as a potential source of noninvasive biomarkers of diseases related to urinary organs (kidney, bladder, urethra, and prostate).Ultracentrifugation is considered the gold standard method for isolation of EVs. However, the precipitates after ultracentrifugation steps are usually contaminated with soluble proteins, such as the Tamm-Horsfall protein (uromodulin).Therefore, ultracentrifugation on a sucrose-deuterium oxide (D
Identifiants
pubmed: 33651361
doi: 10.1007/978-1-0716-1354-2_15
doi:
Substances chimiques
Biomarkers
0
Phosphatidylserines
0
Sucrose
57-50-1
Deuterium Oxide
J65BV539M3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
173-181Références
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