The Design and Preclinical Evaluation of a Single-Label Bimodal Nanobody Tracer for Image-Guided Surgery.
Animals
CHO Cells
Cell Line, Tumor
Cricetulus
Humans
Mice
Neoplasms
/ diagnostic imaging
Radiopharmaceuticals
/ chemistry
Single-Domain Antibodies
/ chemistry
Surgery, Computer-Assisted
Tissue Distribution
Tomography, Emission-Computed, Single-Photon
Tomography, X-Ray Computed
Xenograft Model Antitumor Assays
fluorescence-guided surgery
hybrid imaging
molecular imaging
single-domain antibodies
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
26 02 2021
26 02 2021
Historique:
received:
26
01
2021
revised:
16
02
2021
accepted:
23
02
2021
entrez:
3
3
2021
pubmed:
4
3
2021
medline:
16
9
2021
Statut:
epublish
Résumé
Intraoperative guidance using targeted fluorescent tracers can potentially provide surgeons with real-time feedback on the presence of tumor tissue in resection margins. To overcome the limited depth penetration of fluorescent light, combining fluorescence with SPECT/CT imaging and/or gamma-ray tracing has been proposed. Here, we describe the design and preclinical validation of a novel bimodal nanobody-tracer, labeled using a "multifunctional single attachment point" (MSAP) label, integrating a Cy5 fluorophore and a diethylenetriaminepentaacetic acid (DTPA) chelator into a single structure. After conjugation of the bimodal MSAP to primary amines of the anti-HER2 nanobody 2Rs15d and
Identifiants
pubmed: 33652977
pii: biom11030360
doi: 10.3390/biom11030360
pmc: PMC7996797
pii:
doi:
Substances chimiques
Radiopharmaceuticals
0
Single-Domain Antibodies
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fonds Wetenschappelijk Onderzoek
ID : FWOSB74
Organisme : Stichting Tegen Kanker
ID : FAF-C/2016/813
Organisme : Oncology Research Center of the Universitair Ziekenhuis Brussel
ID : OZR3015
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