CRISPR/Cas9 mutagenesis reveals a role for ABCB1 in gut immune responses to Vibrio diazotrophicus in sea urchin larvae.
Vibrio
ABC transporter
ABCB1
CRISPR/Cas9
Gastrulation
Gut epithelial immunity
IL-17
Marine larvae
Sea urchin
Journal
The Journal of experimental biology
ISSN: 1477-9145
Titre abrégé: J Exp Biol
Pays: England
ID NLM: 0243705
Informations de publication
Date de publication:
01 04 2021
01 04 2021
Historique:
received:
28
06
2020
accepted:
08
02
2021
pubmed:
4
3
2021
medline:
10
7
2021
entrez:
3
3
2021
Statut:
ppublish
Résumé
The ABC transporter ABCB1 plays an important role in the disposition of xenobiotics. Embryos of most species express high levels of this transporter in early development as a protective mechanism, but its native substrates are not known. Here, we used larvae of the sea urchin Strongylocentrotus purpuratus to characterize the early life expression and role of Sp-ABCB1a, a homolog of ABCB1. The results indicate that while Sp-ABCB1a is initially expressed ubiquitously, it becomes enriched in the developing gut. Using optimized CRISPR/Cas9 gene editing methods to achieve high editing efficiency in the F0 generation, we generated ABCB1a crispant embryos with significantly reduced transporter efflux activity. When infected with the opportunistic pathogen Vibrio diazotrophicus, Sp-ABCB1a crispant larvae demonstrated significantly stronger gut inflammation, immunocyte migration and cytokine Sp-IL-17 induction, as compared with infected control larvae. The results suggest an ancestral function of ABCB1 in host-microbial interactions, with implications for the survival of invertebrate larvae in the marine microbial environment.
Identifiants
pubmed: 33653719
pii: 239061
doi: 10.1242/jeb.232272
pmc: PMC8077557
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIEHS NIH HHS
ID : F32 ES029843
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES027921
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES030318
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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