ChIP-Seq from Limited Starting Material of K562 Cells and
Easy workflow
High Signal-to-Noise ratio
High reproducibility
Low Input ChIP-Seq
Tagmentation
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Feb 2020
20 Feb 2020
Historique:
received:
15
08
2019
revised:
16
12
2019
accepted:
23
12
2019
entrez:
3
3
2021
pubmed:
4
3
2021
medline:
4
3
2021
Statut:
epublish
Résumé
Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as 100 human or 1,000
Identifiants
pubmed: 33654745
doi: 10.21769/BioProtoc.3520
pii: e3520
pmc: PMC7842548
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3520Informations de copyright
Copyright 2020 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no financial and non-financial competing interest.
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