Immunoprecipitation and Sequencing of Acetylated RNA.
Epitranscriptome
N4-acetylcytidine
ac4C
acRIP-seq
mRNA modifications
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Jun 2019
20 Jun 2019
Historique:
received:
29
03
2019
revised:
22
05
2019
accepted:
23
05
2019
entrez:
3
3
2021
pubmed:
20
6
2019
medline:
20
6
2019
Statut:
epublish
Résumé
Generation of the epitranscriptome through chemical modifications of protein-coding messenger RNAs (mRNAs) has emerged as a new mechanism of post-transcriptional gene regulation. While most mRNA modifications are methylation events, a single acetylated ribonucleoside has been described in eukaryotes, occurring at the N4-position of cytidine (N4-acetylcytidine or ac4C). Using a combination of antibody-based enrichment of acetylated regions and deep sequencing, we recently reported ac4C as a novel mRNA modification that is catalyzed by the N-acetyltransferase enzyme NAT10. In this protocol, we describe in detail the procedures to identify acetylated mRNA regions transcriptome-wide using acetylated RNA immunoprecipitation and sequencing (acRIP-seq).
Identifiants
pubmed: 33654795
doi: 10.21769/BioProtoc.3278
pii: e3278
pmc: PMC7854089
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3278Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing interests.
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