Lipid-exchange Rate Assay for Lipid Droplet Fusion in Live Cells.
Fluorescence recovery after photobleaching
Lipid droplet fusion
Lipid droplet size
Lipid metabolism
Lipid-exchange rate
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Jul 2019
20 Jul 2019
Historique:
received:
15
03
2019
revised:
13
06
2019
accepted:
14
06
2019
entrez:
3
3
2021
pubmed:
20
7
2019
medline:
20
7
2019
Statut:
epublish
Résumé
Lipid droplets (LDs) are central organelles in maintaining lipid homeostasis. Defective LD growth often results in the development of metabolic disorders. LD fusion and growth mediated by cell death-inducing DNA fragmentation factor alpha (DFFA)-like effector (CIDE) family proteins are crucial for various biological processes including unilocular LD formation in the adipocytes, lipid storage in the liver, milk lipid secretion in the mammary epithelia cells, and lipid secretion in the skin sebocytes. Previous methodology by Gong
Identifiants
pubmed: 33654819
doi: 10.21769/BioProtoc.3309
pii: e3309
pmc: PMC7854171
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3309Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no conflicts of interest or competing interests.
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