Photoaffinity Labeling of Respiratory Complex I in Bovine Heart Submitochondrial Particles by Photoreactive [
Amilorides
Bioenergetics
Chemical biology
Enzyme inhibitor
Mitochondria
NADH-quinone oxidoreductase
Photoaffinity labeling
Respiratory complex I
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Sep 2019
05 Sep 2019
Historique:
received:
14
05
2019
revised:
26
07
2019
accepted:
28
07
2019
entrez:
3
3
2021
pubmed:
5
9
2019
medline:
5
9
2019
Statut:
epublish
Résumé
The architecture of quinone/inhibitor-access channel in proton-translocating NADH-quinone oxidoreductase (respiratory complex I) was modeled by X-ray crystallography and cryo-EM, however, it remains debatable whether the channel model reflects the physiologically relevant state present throughout the catalytic cycle. Using photoreactive [
Identifiants
pubmed: 33654851
doi: 10.21769/BioProtoc.3349
pii: e3349
pmc: PMC7854149
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3349Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no conflicts of interest with the contents of this article.
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