Production of Quantum Dots-containing Influenza Virus Particles for Studying Viral Uncoating Processes.
Influenza A virus
Quantum dots
Uncoating
Viral assembly
vRNP
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Sep 2019
20 Sep 2019
Historique:
received:
25
04
2019
revised:
12
08
2019
accepted:
14
08
2019
entrez:
3
3
2021
pubmed:
20
9
2019
medline:
20
9
2019
Statut:
epublish
Résumé
The genome of influenza A virus (IAV) comprises eight pinlike genomic segments called vRNPs enclosed in viral capsid. During infection, uncoating is the key step for viral replication and represents an antiviral therapeutic target, but it is difficult to observe the transient and dynamic event in detail. Here, we report a protocol for production of quantum dots-containing influenza virus particles by encapsulating quantum dot-conjugated vRNPs during viral assembly. These labeled virions can be used for monitoring viral trafficking in real time and studying viral uncoating processes.
Identifiants
pubmed: 33654863
doi: 10.21769/BioProtoc.3366
pii: e3366
pmc: PMC7854234
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3366Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they do not have any conflicts of interests or competing interests.
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