Isolation and Transcriptomic Profiling of Single Myofibers from Mice.
Non-coding RNAs
Regulatory network
Single myofiber
Skeletal muscle
Transcriptomic analysis
miRNAs
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Oct 2019
05 Oct 2019
Historique:
received:
28
06
2019
revised:
20
08
2019
accepted:
21
08
2019
entrez:
3
3
2021
pubmed:
5
10
2019
medline:
5
10
2019
Statut:
epublish
Résumé
Skeletal muscle is composed of different cells and myofiber types, with distinct metabolic and structural features. Generally, transcriptomic analysis of skeletal muscle is performed using whole muscle, resulting in average information as all cells composing the organ contribute to the expression value detected for each gene with the loss of information about the distinctive features of each specific myofiber type. Since myofibers are the smallest complete contractile system of skeletal muscle influencing its contraction velocity and metabolism, it would be beneficial to have fiber-specific information about gene expression. Here, we describe a protocol for the isolation and the transcriptomic analysis of single individual myofibers. The protocol was set up using single myofibers isolated from
Identifiants
pubmed: 33654874
doi: 10.21769/BioProtoc.3378
pii: e3378
pmc: PMC7853945
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3378Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing interests.
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