Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation.
Fe3O4
SiO2
Bead synthesis
Carboxyl-coating
Magnetic beads
Magnetic nanoparticles
Magnetic separation
Methacrylic acid
Silica-coating
Surface functionalization
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Oct 2019
20 Oct 2019
Historique:
received:
28
05
2019
revised:
18
08
2019
accepted:
10
09
2019
entrez:
3
3
2021
pubmed:
20
10
2019
medline:
20
10
2019
Statut:
epublish
Résumé
The purification of nucleic acids is one of the most common procedures employed in modern molecular biology laboratories. Typically, commercial column-based protocols are utilized to isolate DNA or RNA from various sources. However, these methods not only require specialized equipment, but are also extremely expensive for high-throughput applications. Although an elegant answer to this issue can be provided by paramagnetic beads, bead-based open-source protocols have been limited in the past. Here, we provide an easy to follow step-by-step manual for the synthesis of paramagnetic beads, as well as their functionalization with either a silica- or a carboxyl-surface that can be used to replace the commercial columns with self-made magnetic beads. Together with a variety of detailed protocols for their use in high-throughput nucleic acids extractions, this bead synthesis method forms the recently published open platform Bio-On-Magnetic-Beads (BOMB), which is available on PLOS Biology ( Oberacker
Identifiants
pubmed: 33654895
doi: 10.21769/BioProtoc.3394
pii: e3394
pmc: PMC7853948
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e3394Informations de copyright
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no conflict of interest.
Références
Nanomedicine (Lond). 2014 Mar;9(3):469-85
pubmed: 24646020
Nucleic Acids Res. 1995 Nov 25;23(22):4742-3
pubmed: 8524672
Genome Res. 2012 May;22(5):939-46
pubmed: 22267522
Artif Cells Nanomed Biotechnol. 2016;44(2):722-34
pubmed: 25435409
Nucleic Acids Res. 1994 Oct 25;22(21):4543-4
pubmed: 7971285
Chem Commun (Camb). 2007 Apr 28;(16):1644-6
pubmed: 17530087
PLoS Biol. 2019 Jan 10;17(1):e3000107
pubmed: 30629605
Sci Technol Adv Mater. 2015 Apr 28;16(2):023501
pubmed: 27877761
Biomacromolecules. 2007 Jan;8(1):215-22
pubmed: 17206810